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Live-Cell SICM Imaging: An Introductory Guide for New Users
Martina Papa1,2, François Treussart2, Jean-Pierre Mothet2
1École Polytechnique, Institut Polytechnique de Paris, CNRS, LPICM, 91128 Palaiseau, France.
Abstract:
High-resolution minimally invasive imaging of live cells is essential for investigating cellular morphology and its dynamic changes. Scanning ion conductance microscopy (SICM) offers a unique combination of precise topographic imaging down to the nanoscale with experimental conditions that preserve realistic live-cell behavior. This practical guide walks readers through critical aspects including instrumentation, probe characterization, cell preparation and morphometric data extraction and presentation, equipping them to implement SICM with confidence and begin exploring live-cell structure and dynamics.
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