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Related Experiment Video

Updated: Jul 12, 2026

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Published on: September 6, 2017

Intraprocedural Yield Estimation Using Interim Product CD34+ Concentration Improves the Prediction Accuracy in

Kazuya Okada^1,2, Mayumi Murata¹, Yoshihiro Yabuta¹

¹Transfusion and Hemapheresis Center, Kurashiki Central Hospital, Kurashiki, Japan.

Therapeutic Apheresis and Dialysis : Official Peer-Reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy

|July 9, 2026

Summary

Accurate prediction of CD34+ cell yield using interim apheresis data reduces processed blood volume, minimizing donor burden. This method improves yield estimation for optimized stem cell collection.

Keywords:

CD34 allogeneic apheresis peripheral blood stem cell harvest prediction

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Published on: January 10, 2025

Area of Science:

Hematology
Transplant Immunology
Apheresis Technology

Background:

Accurate prediction of final CD34+ cell yield is crucial for optimizing allogeneic peripheral blood stem cell (PBSC) harvests.
Reducing processed blood volume (PBV) during apheresis can decrease donor burden while ensuring adequate stem cell collection.
Current prediction methods may lack the precision needed for efficient PBV management.

Purpose of the Study:

To compare the accuracy of CD34+ cell yield predictions using pre-procedure versus interim apheresis product measurements.
To evaluate the feasibility of optimizing PBV during apheresis based on interim CD34+ cell concentration.
To identify factors associated with significant decreases in CD34+ cell concentration during apheresis.

Main Methods:

Retrospective analysis of 86 apheresis procedures in 81 healthy donors.
Comparison of prediction errors between pre-procedure peripheral blood CD34+ cell concentration and interim collection-product CD34+ cell concentration (at ~50 mL/kg PBV).
Evaluation of CD34 percent change (CD34% change) and associated factors, including pre-procedure platelet count.

Main Results:

Interim CD34+ cell-based prediction demonstrated significantly smaller absolute prediction error compared to pre-procedure prediction (p=0.034).
Median CD34% change was -3.6%, with 27.9% of procedures showing a decrease of 10% or more.
Higher pre-procedure platelet count was associated with a greater decrease in CD34+ cell concentration during apheresis.

Conclusions:

Interim CD34+ cell concentration measurement during apheresis provides more accurate yield estimations.
This improved prediction accuracy may allow for optimized PBV management, potentially reducing apheresis duration and donor exposure.
The findings support the use of real-time monitoring during apheresis to enhance stem cell collection efficiency and donor safety.