Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A 3-D whole-body human thermoregulatory model to simulate extreme cold stress.

Scientific reports·2026
Same author

Characterization of Workloads Across Three Seasons in Elite Division I Collegiate Women's Basketball Players.

Journal of strength and conditioning research·2026
Same author

The Adaptive Spiral of Collegiate Basketball Performance: A Multiseason Framework Integrating Load, Recovery, and Return-to-Performance.

Journal of strength and conditioning research·2026
Same author

International multisite implementation of distributed cell-free protein biomanufacturing to advance health and research equity.

Science advances·2026
Same author

National Trends in Athletic Training Service Provision in U.S. High Schools: A Five-Year Longitudinal Assessment.

Current sports medicine reports·2026
Same author

National Athletic Trainers' Association Task Force: The Athletic Trainers' Role in Implementing Heat Stress Mitigation Strategies in the Occupational Setting.

Journal of athletic training·2026

Related Experiment Video

Updated: Jul 12, 2026

Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers
08:25

Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers

Published on: December 18, 2013

Sample processing methods affect salivary metabolomics in human exercise-stress studies.

Staci Thornton1, Michael R Szymanski1, Gabrielle J Brewer1

  • 1Human Performance Laboratory, Department of Kinesiology, University of Connecticut, 2095 Hillside Road, U-1110, Storrs, CT, 06269, USA.

Metabolomics : Official Journal of the Metabolomic Society
|July 9, 2026
PubMed
Summary
This summary is machine-generated.

Saliva processing methods impact metabolomics results, but adding steps did not improve metabolite detection or assay accuracy. Standardized methods are crucial for reproducible saliva biomarker research.

Keywords:
Differential abundanceFiltrationHeat stressMetabolomicsMucinsSaliva

More Related Videos

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature
05:33

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature

Published on: June 19, 2019

Assessing the Effects of Music Listening on Psychobiological Stress in Daily Life
07:17

Assessing the Effects of Music Listening on Psychobiological Stress in Daily Life

Published on: February 2, 2017

Related Experiment Videos

Last Updated: Jul 12, 2026

Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers
08:25

Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers

Published on: December 18, 2013

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature
05:33

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature

Published on: June 19, 2019

Assessing the Effects of Music Listening on Psychobiological Stress in Daily Life
07:17

Assessing the Effects of Music Listening on Psychobiological Stress in Daily Life

Published on: February 2, 2017

Area of Science:

  • Biomarker discovery
  • Metabolomics
  • Salivary diagnostics

Background:

  • Saliva biomarker research necessitates understanding how collection and processing influence results.
  • Investigating processing and storage effects is key for accurate stress response and adaptation interpretations.
  • Comparing saliva biomarkers to tissue and blood responses informs understanding of physiological stress.

Purpose of the Study:

  • To evaluate the impact of various saliva processing techniques on metabolomics outcomes.
  • To provide insights for optimizing future experimental designs in salivary biomarker studies.

Main Methods:

  • Assessed effects of centrifugation, time-of-day collection, filtration, and mucinase treatments.
  • Quantified differences in metabolomics results based on varied processing protocols.

Main Results:

  • Additional processing steps did not enhance the number of detected metabolites.
  • No improvement in the sensitivity or specificity of metabolomics results was observed with added steps.

Conclusions:

  • Standardized and comprehensive method descriptions are essential for robust interpretation.
  • Improved reporting will enhance reproducibility in saliva biomarker research.