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Updated: Jul 12, 2026

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
Published on: August 19, 2013
A pyrene-based fluorescent probe for highly sensitive and selective detection of hydroxylamine in environmental water
Wen-Jie Zhang1, Yi Hu1, Yan-Chan Huang1
1School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046 China zwj9007@163.com biwenzhu2018@hactcm.edu.cn.
Abstract:
In this study, a pyrene-based fluorescent probe (PCPA) was designed, synthesized and developed for the detection of HA. In the presence of HA, PCPA was converted to the oxime derivative (PCO) through a nucleophilic addition-elimination reaction, accompanied by significant enhancement in fluorescence at 408/425 nm. Further investigations showed that PCPA displayed high sensitivity (LOD = 0.017 µM, λ ex = 365 nm) within 20 min and high selectivity over other analytes. Moreover, PCPA was capable of recognizing HA in environmental water samples and showed a recovery rate of 95-104% and relative standard deviations (RSD) of 0.56-2.59%. Portable PCPA-loaded paper-based test strips were facilely prepared, which enabled the on-site detection of HA with the aid of a smartphone. These results are expected to make PCPA a potential alternative for HA detection in the field of environmental monitoring.
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