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Updated: Jul 12, 2026

Live Imaging of GFP-labeled Proteins in Drosophila Oocytes

Live Imaging of GFP-labeled Proteins in Drosophila Oocytes

Published on: March 29, 2013

Visualizing Membrane Nanotube Dynamics in Drosophila Oocyte Using Live-Cell Imaging.

Banhisikha Saha^1,2, Sayan Acharjee¹, Jayeeta Nandi¹

¹Department of Biological Sciences, Indian Institute of Science Education & Research- Kolkata, Mohanpur Campus, Mohanpur, Nadia, West Bengal, India.

|July 10, 2026

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Researchers developed a new live-cell imaging method to observe cellular protrusions in developing fruit fly eggs. This technique improves understanding of how these vital structures form and function in real-time.

Area of Science:

Cell Biology
Developmental Biology
Microscopy

Background:

Thin membrane protrusions like cytonemes and nanotubes are crucial for cell communication and development.
The formation and function of these cellular structures are not fully understood.
Existing methods often fail to capture dynamic processes in living tissues.

Purpose of the Study:

To develop an improved live-cell imaging protocol for observing polar cell protrusions in developing *Drosophila* eggs.
To enable real-time, high-resolution tracking of membrane protrusion dynamics.
To provide a foundation for functional studies of membrane protrusions.

Main Methods:

Optimized live-cell imaging combining precise developmental staging, fine dissection, and ex vivo culture of *Drosophila* egg chambers.

Keywords:

Arthropoda Drosophila Germline development MT-Nanotube Micropyle Oogenesis Polar cells

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Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos

Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos

Published on: December 13, 2021

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Last Updated: Jul 12, 2026

Live Imaging of GFP-labeled Proteins in Drosophila Oocytes

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Published on: March 29, 2013

Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos

Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos

Published on: December 13, 2021

Adjusted imaging settings to minimize phototoxicity and thermal stress.

Integrated pharmacological treatments for functional perturbation experiments.

Main Results:

Successfully visualized polar cell nanotube formation and dynamics in real-time within intact living tissue.
Maintained egg chamber viability for extended imaging periods.
Acquired high-resolution, reproducible datasets with minimal artifacts.

Conclusions:

The developed protocol overcomes limitations of fixed-tissue methods for studying membrane protrusions.
This technique provides a robust platform for investigating cell protrusion biology and developmental processes.
The method is adaptable for studying similar phenomena in other biological systems and disease models.