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Lodicule Isolation and Morphometric Analysis During Rice Floret Opening.

Yuan Hu¹, Song Cui¹, Haishen Li¹

¹State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization, Zhongshan Biological Breeding Laboratory, National Observation and Research Station of Rice Germplasm Resources, Nanjing Agricultural University, Nanjing, China.

|July 10, 2026

Summary

Researchers developed a standardized method to quantify rice lodicule (floral organ) morphology changes during flowering. This reproducible workflow aids in understanding lodicule function in floret opening and closure.

Keywords:

Lodicule imaging Lodicule morphometry Morphometric analysis Rice floret Time-course imaging

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Area of Science:

Plant Biology
Reproductive Biology
Morphology

Background:

Rice lodicules are crucial floral organs at the ovary base, driving floret opening and closure through water-mediated swelling and shrinkage.
Quantitative monitoring of lodicule morphological changes is essential for understanding rice reproductive processes, but standardized methods are lacking.

Purpose of the Study:

To establish a detailed and reproducible workflow for quantitative morphometric analysis of rice lodicules.
To provide a standardized framework for evaluating genetic and environmental impacts on lodicule morphology.

Main Methods:

Florets collected at precise time points during flowering.
Lodicules isolated using stereomicroscopy, followed by high-resolution imaging.
Quantitative morphometric analysis of lodicule length, width, and thickness using image analysis software.

Main Results:

A standardized protocol for lodicule sampling, dissection, imaging, and analysis was successfully developed.
The workflow ensures positional consistency, uniform imaging, and standardized data analysis for enhanced reproducibility.
The method effectively captures temporal morphological changes associated with rice floret opening and closure.

Conclusions:

This protocol provides a reliable method for quantitative morphometric analysis of rice lodicules.
It enables accurate assessment of lodicule morphology under varying genetic backgrounds and environmental conditions.
The standardized approach facilitates deeper insights into the role of lodicules in rice reproduction.