Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 12, 2026

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
06:48

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis

Published on: May 31, 2020

Sequential Penta-Omic Extraction Method Using Single Biospecimens of Post-mortem Human Brain.

Scott P Lyons, Brandie M Ehrmann, Thomas S Webb

    Biorxiv : the Preprint Server for Biology
    |July 10, 2026
    PubMed
    Summary

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A Human Pharmacogenomics Approach Provides Insight Into the Pathogenesis and Pathophysiology of Steroid-Induced Ocular Hypertension.

    Ophthalmology science·2026
    Same author

    No evidence for direct physical interaction of 5-HT <sub>2A</sub> -mGluR2 receptors <i>in vitro</i> or <i>in vivo</i>.

    bioRxiv : the preprint server for biology·2026
    Same author

    Acquisition of resistance to RAS inhibition is associated with the upregulation of macropinocytosis through both PI3K-dependent and -independent signaling.

    Cancer research communications·2026
    Same author

    Genome wide association study meta-analysis of neuropathologic lesions of Alzheimer's disease and related dementias in a multi-site autopsy cohort.

    PLoS genetics·2026
    Same author

    Small molecule activators of the mitochondrial protease ClpP induce senescence in triple-negative breast cancer cells and sensitize cells to the Bcl-2 inhibitor venetoclax.

    Cell death & disease·2026
    Same author

    Sequence-encoded differences in the conformational ensembles of CITED transcriptional activation domains impact coactivator binding.

    Protein science : a publication of the Protein Society·2026

    This study introduces a novel penta-omic sequential extraction method (SiMPL-DREx) for analyzing single biospecimens, crucial for precious brain tissue. The method efficiently preserves omic profiles and tissue integrity, minimizing sample loss.

    Area of Science:

    • Neuroscience
    • Genomics
    • Proteomics
    • Metabolomics
    • Lipidomics

    Background:

    • Multi-omic analysis of single biospecimens minimizes heterogeneity and conserves precious samples.
    • Post-mortem human brain microanatomy presents unique challenges for multi-omic studies.
    • Efficient extraction methods are vital for high-quality omic data.

    Purpose of the Study:

    • To develop and validate a penta-omic sequential extraction method for post-mortem human brain biospecimens.
    • To compare the novel method against gold-standard single-omic techniques.
    • To quantify sample loss during the multi-omic extraction process.

    Main Methods:

    • A novel penta-omic sequential extraction method, SiMPL-DREx (Simultaneous Metabolomic, Proteomic, Lipidomic - DNA, RNA Extraction), was applied to post-mortem human brain samples.

    More Related Videos

    Lipidomics and Transcriptomics in Neurological Diseases
    09:58

    Lipidomics and Transcriptomics in Neurological Diseases

    Published on: March 18, 2022

    Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections
    07:55

    Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections

    Published on: November 8, 2024

    Related Experiment Videos

    Last Updated: Jul 12, 2026

    On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
    06:48

    On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis

    Published on: May 31, 2020

    Lipidomics and Transcriptomics in Neurological Diseases
    09:58

    Lipidomics and Transcriptomics in Neurological Diseases

    Published on: March 18, 2022

    Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections
    07:55

    Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections

    Published on: November 8, 2024

  • Omic extracts were compared to those from gold-standard single-omic methods.
  • Tissue integrity (RIN) and omic profiles were assessed for all extracts.
  • Solvent percentages and upstream losses were quantified for aqueous and organic phases.

    • Main Results:

    • The SiMPL-DREx method yielded omic profiles and tissue integrity numbers comparable to gold-standard single-omic methods.
    • No significant differences were observed in omic profiles or tissue integrity.
    • The study confirmed an omics loss of under 5% for the sequential extraction process.

    Conclusions:

    • The SiMPL-DREx method provides a robust and efficient approach for multi-omic analysis of limited biospecimens, particularly post-mortem brain tissue.
    • This method overcomes limitations of previous multi-omic studies by quantifying losses and ensuring data quality.
    • SiMPL-DREx facilitates comprehensive molecular profiling from a single precious sample, advancing neuroscience research.