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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

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Related Experiment Video

Updated: Jul 12, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Monitoring microscope performance in an imaging facility using OMERO-metrics.

Sophia Sommer, Oumou Dhmine, Julio Mateos Langerak

    Biorxiv : the Preprint Server for Biology
    |July 10, 2026
    PubMed
    Summary
    This summary is machine-generated.

    Regularly testing microscope performance using fluorescent beads helps ensure accurate bioscience research. This method quanties image resolution and detects instrument degradation, safeguarding data quality over time.

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    Last Updated: Jul 12, 2026

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    Area of Science:

    • Bioscience and optical microscopy
    • Biotechnology and molecular imaging

    Background:

    • Optical microscopy is crucial for bioscience discoveries, but instrument degradation can impact data quality.
    • Immuno-fluorescence and molecular biology techniques have advanced optical microscopy capabilities.

    Purpose of the Study:

    • To develop and validate workflows for assessing microscope performance and detecting instrument issues.
    • To establish a reliable method for measuring image resolution and identifying degradation over time.

    Main Methods:

    • Utilizing fluorescent beads to record the point spread function (PSF) and measure microscope resolution.
    • Preparing beads, imaging them as 3D Z-stacks, uploading data to OMERO, and processing with OMERO-metrics.

    Main Results:

    • Successfully developed protocols for routine microscope performance testing.
    • Demonstrated the ability of the method to detect significant instrument degradation and issues.
    • Quantified image resolution using fluorescent bead PSF measurements.

    Conclusions:

    • Regular instrument testing using fluorescent beads is essential for maintaining data integrity in bioscience.
    • The developed OMERO-metrics workflow provides a robust solution for monitoring microscope performance.
    • Proactive detection of instrument issues prevents compromised research outcomes.