Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

R1398 is the GTP-γ-phosphate sensor that drives the ROC G-domain switching mechanism unique to Parkinson's disease-associated LRRK2.

Research square·2026
Same author

Cytoplasmic lattices store developmentally poised degradative and cytoskeletal complexes in mammalian eggs.

Nature structural & molecular biology·2026
Same author

Functional traits mediate the effects of soil property on the growth performance of Ardisia gigantifolia cuttings.

BMC plant biology·2026
Same author

OGT-mediated PIN O-GlcNAcylation drives depression-like behaviors by impairing NOS-stargazin-GluA1 signaling.

Communications biology·2026
Same author

Predictive value of peripheral lymphocyte subsets in relation to opportunistic infections in people living with HIV/AIDS.

BMC infectious diseases·2026
Same author

Lattice-Induced 2D Ti<sub>3</sub>C<sub>2</sub>T<sub><i>x</i></sub> MXene and COF Supramolecular Nanohybrids for Electrocatalytic Water Splitting.

Langmuir : the ACS journal of surfaces and colloids·2026

Related Experiment Video

Updated: Jul 12, 2026

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
11:55

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Published on: May 28, 2021

CryoGO enables high-resolution structural profiling of endogenous cellular macromolecules.

Yujie Li1, Yuekang Zhang1, Chunxiang Wu1

  • 1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA.

Biorxiv : the Preprint Server for Biology
|July 10, 2026
PubMed
Summary

A new method called cryoGO mechanically opens cells on EM grids, enabling high-resolution cryo-electron microscopy of native cellular structures. This rapid technique provides insights into macromolecular assemblies and cellular dynamics.

More Related Videos

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
09:25

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis
08:26

Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis

Published on: December 10, 2019

Related Experiment Videos

Last Updated: Jul 12, 2026

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
11:55

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Published on: May 28, 2021

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
09:25

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis
08:26

Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis

Published on: December 10, 2019

Area of Science:

  • Structural Biology
  • Cell Biology
  • Cryo-electron Microscopy

Background:

  • Understanding cellular function requires visualizing macromolecular structures in their native environment.
  • Current methods for preparing cellular specimens for high-resolution imaging are limited in accessibility and throughput.
  • Bridging the gap between molecular architecture and physiological function remains a challenge.

Purpose of the Study:

  • To introduce a novel, rapid, and scalable method for preparing cell-derived specimens for cryo-electron microscopy.
  • To enable high-resolution structure determination of endogenous macromolecular assemblies within their native cellular context.
  • To facilitate time-resolved structural profiling of cellular processes.

Main Methods:

  • cryoGO (on-Grid Opening cryo-electron microscopy) mechanically disrupts cells directly on EM grids.
  • The method produces cell-derived specimens suitable for high-resolution single-particle cryo-EM.
  • Compatible with standard cryo-EM infrastructure and requires minimal cell numbers.

Main Results:

  • Achieved near-atomic resolution structure determination of diverse endogenous macromolecular assemblies.
  • Captured the conformational and compositional landscape of ribosomes, preserving cellular state distributions and spatial heterogeneity.
  • Demonstrated the capability for time-resolved structural profiling on timescales of seconds.

Conclusions:

  • cryoGO significantly lowers the technical barrier to high-resolution native structural biology.
  • The method provides unprecedented insights into the structural dynamics and heterogeneity of cellular components.
  • Enables rapid structural profiling for studying cellular adaptation and remodeling.