Preparation of Optical-Based Sensors for the Determination of Cardiac Myosin-Binding Protein C

In this study, cardiac myosin-binding protein-C (cMyBP-C) imprinted and nonimprinted poly-(2-hydroxyethyl methacrylate-N-methacryloxy-(L)-tryptophan) polymeric film-based surface plasmon resonance (SPR) sensors were prepared for the detection of cMyBP-C using the microcontact imprinting method. The surface characterization of cMyBP-C imprinted (MIP) and nonimprinted (NIP) SPR sensors was investigated using contact angle measurements and atomic force microscopy. Kinetic analyses were performed in the concentration range of 0.05-300 ng/mL, and the limits of detection and quantification were calculated as 0.019 and 0.064 ng/mL, respectively. Equilibrium, adsorption, and desorption cycles were completed for 10 min in all of the kinetic analyses. The selectivity studies of MIP SPR and NIP SPR sensors were performed using the template molecule cMyBP-C with competing molecules creatine kinase-MB (CK-MB) and cardiac troponin T (cTnT). The relative selectivity coefficients of the MIP SPR sensor for cMyBP-C/cTnT and cMyBP-C/CK-MB were found to be 3.85 and 12.64 times, respectively. The applicability of the MIP SPR sensor was determined using solutions prepared in artificial plasma samples, both in the SPR system and by enzyme-linked immunosorbent assay (ELISA).