Interfacial Charge Separation Engineering-Modulated Multichannel Photoelectrochemical Biosensor Enabled by a...

Area of Science:

• Materials Science
• Nanotechnology
• Analytical Chemistry

Background:

• Accurate biomarker detection is critical for early disease diagnosis and effective treatment.
• Existing diagnostic methods may lack the sensitivity or specificity required for early-stage disease identification.
• Multiplexed detection strategies offer enhanced diagnostic capabilities by analyzing multiple targets simultaneously.

Purpose of the Study:

• To develop a novel multiplexed biosensor for simultaneous electrochemical (EC) and photoelectrochemical (PEC) detection of bovine serum albumin (BSA).
• To utilize self-templated In2S3/CuInS2 (CIS) heterostructured nanotubes for enhanced biosensing performance.
• To integrate molecular imprinting technology for specific recognition and highly sensitive detection of BSA.

Main Methods:

• Fabrication of In2S3/CuInS2 (CIS) heterostructured nanotubes via a self-templating method.
• Simultaneous EC and PEC measurements for BSA detection.
• Utilizing differential pulse voltammetry (DPV) for EC detection and photocurrent analysis for PEC detection.
• Incorporating molecular imprinting to enhance selectivity for BSA.

Main Results:

• The In2S3/CIS heterostructure demonstrated high sensitivity in both EC and PEC modes.
• The biosensor achieved a wide linear detection range for BSA from 10^-20 to 10^-1 mg·mL^-1.
• A remarkably low detection limit of 8.5 × 10^-21 mg·mL^-1 for BSA was achieved.
• The integrated molecular imprinting significantly improved the specificity of BSA detection.

Conclusions:

• The developed multiplexed biosensor offers a universal and efficient platform for sensitive and selective BSA detection.
• This technology holds significant promise for applications in clinical biomedicine and bioanalysis.
• The combination of EC, PEC, and molecular imprinting provides a powerful strategy for advanced biomarker detection.