Related Experiment Video
Updated: Jul 12, 2026

Biogas Purification through the use of a Microalgae-Bacterial System in Semi-Industrial High Rate Algal Ponds
Published on: March 22, 2024
Activated Microalgae-Activated MLSS Bacteria Consortium Synergistically Improves Phycoremediation Kinetics: A
Geethanjali Udaya Kumar1, Lazar Mukilan Antony1, Judah Jacob1
1Department of Microbiology, Madras Christian College (Affiliated to the University of Madras), Chennai, India.
A novel bioactivation approach using Chlorella vulgaris and bacteria enhanced wastewater treatment. This integrated system achieved high nutrient removal and BOD reduction, showcasing sustainable biological treatment potential.
Area of Science:
- Environmental Microbiology
- Aquatic Ecology
- Biotechnology
Background:
- Sustainable wastewater treatment requires efficient nutrient removal and resource recovery.
- Integrated biological systems combining microalgae and bacteria offer a promising solution.
- High-rate algal ponds (HRAPs) are effective for wastewater treatment but can be optimized.
Purpose of the Study:
- To develop and evaluate a novel parallel bioactivation approach for wastewater treatment.
- To investigate the synergistic effects of Chlorella vulgaris and a mixed liquor suspended solids (MLSSs) bacterial consortium.
- To assess the efficiency of nutrient remediation and biochemical oxygen demand (BOD) reduction in an outdoor HRAP.
Main Methods:
- Parallel bioactivation of Chlorella vulgaris in suboptimal nutrient conditions.
- Integration of separately activated bacterial MLSS with activated Chlorella vulgaris to form an AM-AB consortium.
- Application of the AM-AB consortium in an outdoor high-rate algal pond (HRAP) for wastewater treatment.
Main Results:
- Achieved 99.9% nitrogen removal efficiency within 6 hours and complete phosphate removal in 2 hours.
- Demonstrated a maximum specific removal rate for total nitrogen of 49.81 mg g⁻¹ h⁻¹.
- Attained 81.73% BOD reduction, indicating significant synergistic interaction between microalgae and bacteria.
Conclusions:
- The developed AM-AB consortium shows high efficiency in nutrient remediation and BOD reduction.
- The synergistic interaction between Chlorella vulgaris and bacteria enhances wastewater treatment performance.
- This approach holds significant potential for robust, sustainable, and integrated biological wastewater treatment systems.
Related Concept Videos
Biofuels
Microbial Bioremediation of Hydrocarbons
Microbial Wastewater Treatment
Bioremediation
Microbial Fuel Cells
Green Algae

