Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 12, 2026

Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
09:45

Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium

Published on: May 18, 2021

Computer-Assisted Hapten Design for High-Performance Recombinant Antibody against Napropamide: Molecular Recognition

Huihui Wu1,2, Xinxin Xu1,2, Liguang Xu1,2

  • 1State Key Laboratory of Food Science and Resources, Jiangnan University,Wuxi, Jiangsu 214122, People's Republic of China.

Analytical Chemistry
|July 10, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spin-controlled enantioselective near-infrared photocatalysis with chiral MgO/Co<sub>3</sub>O<sub>4</sub> nanoparticles.

Nature communications·2026
Same author

Guiding point-of-care therapeutic drug monitoring through structure-toxicity principles.

Chemical science·2026
Same author

Response of soil nitrogen-cycling functional genes and their associations to nitrogen enrichment in a typical subtropical estuary (Min River), Southeast China.

Marine pollution bulletin·2026
Same author

Computer-Aided Rational Hapten Design for Broad-Spectrum Monoclonal Antibody Development against Anthraquinones and Its Application in Lateral Flow Immunoassay.

Analytical chemistry·2026
Same author

Rate-Distortion Limits for Task-Oriented Compression with Side Information.

Entropy (Basel, Switzerland)·2026
Same author

Insecticide Resistance Dynamics and Spinosyn Cross-Resistance in <i>Megalurothrips usitatus</i> in Hainan, China.

Insects·2026

A novel computer-assisted design for Napropamide (Nap) detection yielded a highly sensitive recombinant antibody. This antibody enabled a rapid, user-friendly test strip for detecting Nap residues in various food samples.

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Food Science

Background:

  • Napropamide (Nap) is widely used, posing potential health risks.
  • A need exists for rapid and sensitive Nap residue detection methods in food.

Purpose of the Study:

  • To design novel haptens for Nap detection using a computer-assisted strategy.
  • To develop a highly sensitive recombinant antibody (rAb) and a rapid detection tool for Nap residues.

Main Methods:

  • Computer-assisted hapten design (H1 and H2) and molecular docking (MD).
  • Generation and characterization of recombinant antibodies (rAbs).
  • Alanine scanning mutagenesis for validating antibody-target interactions.
  • Development of a dual T-line colloidal gold test strip.

More Related Videos

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Related Experiment Videos

Last Updated: Jul 12, 2026

Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium
09:45

Production of Monoclonal Antibodies Targeting Aminopeptidase N in the Porcine Intestinal Mucosal Epithelium

Published on: May 18, 2021

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Main Results:

  • Hapten H1 generated a high-sensitivity rAb 2A4 (IC50 = 1.64 ng/mL), outperforming hapten H2-derived rAb 3D7 (IC50 = 5.08 ng/mL).
  • Molecular docking and mutagenesis confirmed key interactions responsible for rAb 2A4's high sensitivity.
  • The developed test strip showed a limit of detection of 18.72-36.54 μg/kg for Nap in various food matrices.
  • Results correlated well with Liquid Chromatography-Tandem Mass spectrometry.

Conclusions:

  • Rational hapten design significantly enhances antibody sensitivity for Nap detection.
  • The developed colloidal gold test strip offers a sensitive, user-friendly, and high-throughput method for Nap residue monitoring in food.
  • This study provides a practical tool for ensuring food safety regarding Nap contamination.