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Updated: Jul 12, 2026
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Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
An electrochemical-fluorescence dual-mode biosensor enabled by a self-protected circular DNAzyme walker for kanamycin
Sha Yu1, Kaikai Si1, Jiayue Tian1
1School of Chemistry and Chemical Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, P. R. China. shayu@xauat.edu.cn.
The Analyst
|July 10, 2026
Summary
This study presents a novel dual-mode biosensor for detecting kanamycin (KAN). The biosensor achieves highly sensitive and selective kanamycin detection in complex samples, even enabling real-time imaging in living cells.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Biosensor Technology
Background:
- Accurate detection of kanamycin (KAN) in complex environments is crucial but challenging.
- Existing methods often lack the required sensitivity or specificity for real-world applications.
Purpose of the Study:
- To develop a highly sensitive and selective dual-mode biosensor for kanamycin detection.
- To integrate electrochemical and fluorescence detection for enhanced signal amplification and analysis.
Main Methods:
- Utilized an aptamer for specific kanamycin recognition.
- Employed a gold nanoparticle-based DNAzyme walker for signal amplification.
- Integrated electrochemical and fluorescence detection mechanisms.
Main Results:
- Achieved ultra-low detection limits: 0.018 fM (electrochemical) and 0.327 pM (fluorescence).
- Demonstrated excellent selectivity for kanamycin in complex matrices.
- Enabled *in situ* imaging of kanamycin within living cells using fluorescence.
Conclusions:
- The developed dual-mode biosensor offers a sensitive, selective, and versatile platform for kanamycin detection.
- This technology has potential applications in environmental monitoring and biological studies.
- The dual-mode approach enhances reliability and provides complementary analytical information.
