Related Experiment Video
Updated: Jul 12, 2026
09:33
Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
Published on: March 29, 2018
Immunohistochemistry in placental pathology
1Department of Pathology and Laboratory Medicine, College of Medicine, University of Cincinnati, Cincinnati, United States.
Summary
Immunohistochemistry (IHC) is crucial for diagnosing placental issues in high-risk pregnancies. Specific IHC stains, like E-cadherin/CD34, detect early fetal vascular malperfusion and other placental injuries.
Area of Science:
- Pathology
- Reproductive Medicine
- Immunohistochemistry
Background:
- Standard placental examination often requires advanced techniques for accurate diagnosis.
- High-risk pregnancies necessitate precise placental evaluation to ensure fetal well-being.
Purpose of the Study:
- To highlight the essential role of immunohistochemistry (IHC) in placental diagnosis.
- To detail specific IHC applications in identifying various placental pathologies.
Main Methods:
- Utilizing double E-cadherin/CD34 immunostaining for early fetal vascular malperfusion (FVM).
- Employing cytokeratin, smooth muscle actin, and p57 immunostains for specific diagnostic scenarios.
- Assessing IHC's efficacy in diagnosing viral placentitis.
Main Results:
- The E-cadherin/CD34 stain is highly sensitive for detecting early FVM, characterized by endothelial fragmentation.
- IHC aids in grading FVM, identifying villous hypomaturity, and confirming intrauterine pregnancy.
- Specific stains are valuable for diagnosing hydatidiform moles and viral placentitis.
Conclusions:
- Immunohistochemistry is indispensable for complex placental diagnoses, especially in high-risk pregnancies.
- Specific IHC markers provide critical diagnostic information beyond conventional methods.
- IHC enhances the ability to diagnose and manage fetal vascular malperfusion and other placental disorders.
Related Concept Videos
01:15Transcytosis of IgG
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
01:22Immunocytochemistry and Immunohistochemistry
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
These...