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Updated: Jul 12, 2026

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
Published on: August 19, 2012
Recent advances in bioconjugation via CuAAC click reaction: Synthesis and bio-applications
Anurag Shukla1, Yogesh Yadav1, Ram Sagar1
1Glycochemistry Laboratory, School of Physical Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
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Click chemistry is a highly reliable and straightforward method for bioconjugation. It exhibits high selectivity and biocompatibility, rendering biorthogonal tagging and regenerative medicine. Its transformative applications in targeted therapy, drug delivery, molecular imaging, and nanotechnology are paving the way for groundbreaking advancements in chemistry and biology. In this review, we discuss the detailed application of Copper-catalysed azide-alkyne cycloaddition (CuAAC), with limited examples of SPAAC, IEDDA, SuFEX, SeNEx and PFEx including recent advances in bioconjugation through the application of bioorthognal methods using click chemistry, focusing on the synthesis of privileged bioconjugates during 2020-2025. Thus, this review highlights the significance of click chemistry as a powerful method for bioconjugation, providing a valuable resource for the scientific community on click chemistry and its applications in biomedical sciences.
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