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Effects Of Human Apical Papilla Cell Senescence On Repair-Related Functions In Regenerative Endodontics.

Letícia Odaguiri Watanabe1, Larissa Barbosa de Sousa1, Maria Ester França de Melo1

  • 1Postgraduate Program in Health Sciences, Faculty of Health Sciences, University of Brasília (UnB), Brasília, DF, Brazil.

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Cellular senescence impairs human apical papilla cell (hAPCs) function, reducing migration and proliferation. This senescence-induced change in immunoinflammatory response may impact regenerative endodontic therapy (RET) outcomes.

Keywords:
Cellular SenescenceDental PapillaMesenchymal Stem CellsRegenerative EndodonticsSenescence-Associated Secretory Phenotype

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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Area of Science:

  • Dental research
  • Cell biology
  • Tissue regeneration

Background:

  • Cellular senescence in human apical papilla cells (hAPCs) can negatively affect their function.
  • The specific impact of senescence on hAPC mitochondrial activity, morphology, and behavior is not fully understood.
  • Understanding these effects is crucial for advancing regenerative endodontic therapy (RET).

Purpose of the Study:

  • To investigate the effects of senescence on hAPCs.
  • To evaluate changes in mitochondrial activity, morphology, migration, proliferation, and immunoinflammatory response.
  • To determine the implications for regenerative endodontic therapy.

Main Methods:

  • Senescence was induced in hAPCs, confirmed by SA-β-Gal staining and p53 expression.
  • Cells were stimulated with control medium, lipopolysaccharide (LPS), or LPS plus interferon-gamma (IFN-γ).
  • Assessed mitochondrial activity, morphology, migration, proliferation, and cytokine gene expression (TNF-α, IL-6, IL-10, TGF-β).

Main Results:

  • Senescence increased SA-β-Gal activity and p53 expression.
  • Senescent hAPCs exhibited enlarged morphology and reduced extensions, with impaired migration and proliferation.
  • Pro-inflammatory cytokine IL-6 increased, TGF-β decreased, and IL-10 showed a limited increase under LPS, indicating an altered immunoinflammatory profile.

Conclusions:

  • Cellular senescence significantly compromises key functional properties of hAPCs.
  • Senescence disrupts the immunoinflammatory profile of hAPCs, shifting towards a pro-inflammatory state.
  • These alterations may negatively affect biological processes vital for successful RET.