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Circular dichroism and DNA secondary structure.

W A Baase, W C Johnson

    Nucleic Acids Research
    |February 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    High salt concentrations induce a change in DNA

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    Area of Science:

    • Molecular Biology
    • Biophysics

    Background:

    • DNA's secondary structure is sensitive to ionic strength.
    • The B-form is typical at low salt, while the C-form is observed at high salt concentrations.

    Purpose of the Study:

    • To quantify the change in DNA helix rotation upon transfer to high salt conditions.
    • To correlate Circular Dichroism (CD) intensity with DNA secondary structure changes.

    Main Methods:

    • Spectroscopic analysis of DNA in various salt solutions (NaCl, CsCl, LiCl, NH4Cl).
    • Measurement of Circular Dichroism (CD) at 275 nm.
    • Determination of winding angles and molar extinction coefficients for ethidium.

    Main Results:

    • DNA helix rotation changes by -0.22 base pairs per turn when transferred from 0.05 M NaCl to high salt solutions.

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  • Mononucleosomes in the C-form exhibit an additional -0.31 turns about the histone core.

    • Conclusions:

    • High salt concentrations induce a measurable alteration in DNA's helical geometry, consistent with a transition towards C-form.
    • CD spectroscopy is a reliable method for assessing DNA secondary structure changes in response to environmental conditions.