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Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
Published on: November 21, 2023
Simultaneous detection of multiple foodborne pathogens using a CRISPR/Cas12a-based pump-free microfluidic chip.
Yachen Tian1, Meng Li1, Cheng Liu2
1School of Medicine, Linyi University, Shandong, 276000, People's Republic of China.
Analytical and Bioanalytical Chemistry
|July 10, 2026
Summary
This study presents a CRISPR/Cas12a microfluidic biosensor for rapid, simultaneous detection of four foodborne pathogens. The system achieves high sensitivity and specificity, offering a promising tool for on-site food safety monitoring.
Area of Science:
- Biotechnology
- Food Science
- Analytical Chemistry
Background:
- Microfluidic chips offer efficient nucleic acid detection for food safety.
- CRISPR technology provides high specificity and sensitivity for nucleic acid analysis.
Purpose of the Study:
- To construct a microfluidic biosensor using the CRISPR/Cas12a system.
- To achieve rapid, simultaneous detection of Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O157:H7, and Cronobacter sakazakii.
Main Methods:
- A pump-free microfluidic chip was utilized as the carrier.
- CRISPR/Cas12a system was employed for pathogen detection.
- Dual replicate channels with controls were established for reliability.
Main Results:
- Simultaneous, high-sensitivity, high-specificity, and high-accuracy detection of four foodborne pathogens was achieved.
- Detection limits were as low as 10^2-10^3 CFU/mL for the target bacteria.
- Fluorescence visualization enabled easy interpretation of results.
Conclusions:
- The integrated CRISPR/Cas12a sensor chip enables efficient simultaneous detection of multiple pathogens.
- The biosensor demonstrates parallel verification and control capabilities.
- This technology shows broad application prospects for on-site rapid nucleic acid analysis in food safety.
