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Preparation and evaluation of the effect of hydrogel containing Cedrus and mupirocin and application as a wound
Mohammad B Heydari1,2, Hosna Alvandi3,4, Tahereh Naseriyeh5
1Department of General Surgery, Faculty of Kermanshah University of Medical Science, Kermanshah, Iran.
Background:
Hydrogel wound dressings, which contain antibiotics and natural tissue repair agents, can be a suitable option for wound treatment. Cedrus, due to its antibacterial properties, can significantly promote wound healing. This study aimed to evaluate the effectiveness of a hydrogel containing mupirocin and Cedrus in promoting wound healing and assessing histopathological changes in the skin tissue of adult male rats.
Method:
Hydrogels based on polymer alginate and gelatin were prepared and cross-linked with Genipin and calcium chloride in two steps. Mupirocin and Cedrus were loaded in hydrogels. Animals were randomly divided into four groups of seven rats: negative control group (without treatment), positive control group (phenytoin), hydrogel group (received hydrogel without drug), and hydrogel containing mupirocin and Cedrus (H + C + M) group. The treatment period lasted for 21 days.
Result:
Biological tests showed that the prepared hydrogels are biodegradable, have appropriate swelling power, and are biocompatible. The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide showed that the components of hydrogels did not have a toxic effect on cells and improved the function of cells, their growth, and proliferation. Histopathological examination also showed that in the H + C + M group, compared to the negative control group and hydrogel, skin tissue changes were decreased, epidermis was formed in a wide area, and inflammatory cells were also reduced. Moreover, collagen fibers were formed, which shows the antibacterial properties of mupirocin and the elasticity of the hydrogel.
Conclusion:
Based on the findings, the hydrogel dressing containing Cedrus and mupirocin possesses favorable structural, physical, and biological properties and shows therapeutic potential for skin wound healing.
