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Related Experiment Video

Updated: Jul 13, 2026

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery
05:30

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery

Published on: January 31, 2025

Organ-Derived Spectral Libraries Improve Sensitivity in DIA Plasma Peptidomics.

Yusei Okuda1,2, Ryo Konno2, Tomomi Taguchi3

  • 1Department of Physics, School of Science, Kitasato University, Kanagawa, Japan.

Proteomics
|July 11, 2026
PubMed
Summary

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This study introduces a sensitive plasma peptidomic method combining data-independent acquisition (DIA) with spectral libraries. The approach identifies over 5500 peptides, including 2000 organ-derived ones, aiding disease biomarker discovery.

Area of Science:

  • Biochemistry
  • Proteomics
  • Systems Biology

Background:

  • Plasma peptides are vital for homeostasis and disease regulation.
  • High-abundance proteins like albumin complicate the analysis of organ-secreted peptides in plasma.
  • Existing methods face challenges in comprehensively identifying native organ-derived peptides.

Purpose of the Study:

  • To develop a highly sensitive plasma peptidomic approach for analyzing organ-derived peptides.
  • To combine data-independent acquisition (DIA) with organ and plasma spectral libraries.
  • To enable quantitative analysis and organ-of-origin assignment for plasma peptides.

Main Methods:

  • Peptide extraction from plasma and eleven organ types using the differential solubilization method.
  • Construction of an empirical spectral library via data-dependent acquisition (DDA) on a timsTOF HT.
Keywords:
DIA‐MSmousepeptidomepeptidomicsplasma

Related Experiment Videos

Last Updated: Jul 13, 2026

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery
05:30

Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery

Published on: January 31, 2025

  • Analysis of DIA-MS data from plasma samples utilizing the established spectral library.

    • Main Results:

    • Identification of over 5500 peptides per run on average.
    • Detection of over 2000 organ-derived peptides, including 19 known bioactive peptides.
    • Successful linkage of identified peptides to their secreting organs.

    Conclusions:

    • The novel DIA-MS strategy enables highly sensitive quantification of organ-derived peptides in plasma.
    • This approach facilitates the discovery of novel biomarkers and bioactive peptides.
    • The method is expected to advance the understanding of systemic disease pathophysiology.