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Related Experiment Video

Updated: Jul 13, 2026

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
10:05

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins

Published on: August 7, 2014

Protein Enrichment from Ascites Fluid Using Nanotrap Hydrogel Particle Technology.

Carly A I Twigg1, Kathleen M Madden1, Kristin L M Boylan1

  • 1University of Minnesota, Department of Laboratory Medicine and Pathology, Minneapolis, Minnesota 55455, United States.

Journal of Proteome Research
|July 11, 2026
PubMed
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Nanotrap Protein Enrichment Affinity Kits (PEAK) enhance mass spectrometry detection of low-abundance proteins in ovarian cancer ascites fluid. This cost-efficient method significantly increases identified proteins for biomarker discovery.

Area of Science:

  • Proteomics
  • Biochemistry
  • Oncology

Background:

  • Ascites fluid is a key biological matrix in high-grade serous ovarian carcinoma (HGSOC).
  • Proteomic analysis of ascites fluid requires methods to manage the wide dynamic range of protein concentrations.
  • Previous protein enrichment techniques have not been evaluated for ascites fluid.

Purpose of the Study:

  • To evaluate Nanotrap Protein Enrichment Affinity Kits (PEAK) for enhancing mass spectrometry (MS)-based proteomic analysis of ascites fluid.
  • To assess the ability of Nanotrap PEAK to detect lower-abundance proteins in HGSOC ascites fluid for biomarker discovery.
  • To determine if Nanotrap PEAK is a suitable method for proteomic biomarker discovery in ascites fluid.

Main Methods:

  • Utilized Nanotrap PEAK, comprising magnetic hydrogel nanoparticles (A, B, C) with selective chemistries.
Keywords:
ascitesbiomarkerenrichmentmass spectrometrynanoparticleovarian cancerproteomics

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Last Updated: Jul 13, 2026

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  • Applied Nanotrap PEAK to 10 HGSOC-derived ascites fluid samples.
  • Compared proteomic analysis results from Nanotrap-processed samples against neat (unprocessed) ascites samples using MS.

    • Main Results:

    • Nanotrap PEAK resulted in >2-3 times more identified proteins compared to neat ascites.
    • An average of 61% of proteins were enriched, and 39% were depleted by Nanotrap particles.
    • Identified up to 3480-4000 proteins in Nanotrap-processed samples, versus 1505 in neat samples.

    Conclusions:

    • Nanotrap PEAK effectively enhances the detection of low-abundance proteins in ascites fluid.
    • This method significantly increases proteome coverage for MS-based biomarker discovery in HGSOC.
    • Nanotrap PEAK offers a cost-efficient and scalable approach for ascites fluid proteomic studies.