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Related Experiment Video

Updated: Jul 13, 2026

Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices
04:08

Coherent Anti-Stokes Raman Spectroscopy (CARS) Application for Imaging Myelination in Brain Slices

Published on: July 22, 2022

Label-Free Myelin Fingerprinting: An Adult Human Post-Mortem Brain Slice Platform via Coherent Anti-Stokes Raman

Kasra Roya-Kouchaki1,2,3,4, Jeroen P Korterik4, Femke Tjebbes1,2,3

  • 1Department of Anatomy and Neurosciences, Amsterdam University Medical Center, VU Medical Center, VU University, Amsterdam, The Netherlands.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|July 11, 2026
PubMed
Summary

Human organotypic slice cultures (OSCs) from corpus callosum (CC) tissue offer a novel model for studying myelin disorders like multiple sclerosis (MS). This method, combined with Coherent anti-Stokes Raman spectroscopy (CARS) imaging, analyzes myelin changes within 32 hours post-mortem.

Keywords:
coherent anti‐stokes raman spectroscopymultiple sclerosismyelin

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Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin

Published on: March 26, 2015

Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Biochemistry

Background:

  • Traditional animal and cell models inadequately represent human physiology for neurological research.
  • Human organotypic slice cultures (OSCs) are an emerging alternative for studying human-specific neurological conditions.
  • The corpus callosum (CC) is crucial for myelin research due to its high myelination.

Purpose of the Study:

  • To establish and characterize a human organotypic slice culture (OSC) model using post-mortem corpus callosum (CC) tissue.
  • To investigate the potential of Coherent anti-Stokes Raman spectroscopy (CARS) for label-free molecular analysis of myelin within OSCs.
  • To demonstrate the utility of this model for studying myelin degeneration in diseases like multiple sclerosis (MS).

Main Methods:

  • Culturing post-mortem human corpus callosum (CC) tissue as organotypic slice cultures (OSCs) in a chemically defined, serum-free medium.
  • Performing viability studies to determine the optimal culture duration (at least 8 days in vitro).
  • Utilizing label-free Coherent anti-Stokes Raman spectroscopy (CARS) imaging for molecular analysis of myelin structure and composition.

Main Results:

  • OSCs derived from human CC tissue maintained viability for at least 8 days in vitro.
  • A 32-hour window post-time of death (TOD) was identified where myelin integrity remained stable for baseline measurements.
  • Cultures from multiple sclerosis (MS) patients exhibited altered myelin spectral profiles compared to non-MS controls, demonstrating disease relevance.

Conclusions:

  • The study successfully developed a human myelin model using OSCs and characterized its viability and stability.
  • Coherent anti-Stokes Raman spectroscopy (CARS) imaging provides a powerful tool for label-free molecular analysis of myelin in this model.
  • This novel toolset holds significant promise for future research into myelin-based neurological disorders, including multiple sclerosis (MS).