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Related Experiment Video

Updated: Jul 13, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

High-efficiency 14-plex cSNP SNaPshot assay for forensic peripheral blood identification.

Yixia Zhao1, Yuting Sun2, Siyi Wei2

  • 1Institute of Evidence Law and Forensic Science, China University of Political Science and Law, Beijing, 100088, China.

BMC Genomics
|July 11, 2026
PubMed
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Forensic scientists can now identify bloodstains, even in mixed samples, using a new multiplex assay targeting 14 blood mRNA biomarkers. This method enhances crime scene analysis by differentiating body fluids with high accuracy.

Area of Science:

  • Forensic Science
  • Molecular Biology
  • Genetics

Background:

  • Accurate identification of body fluid stains at crime scenes is crucial for forensic investigations.
  • Distinguishing between different body fluids, especially in mixed stains, presents a significant challenge.
  • Messenger RNA (mRNA) biomarkers offer a promising avenue for differentiating forensic body fluids.

Purpose of the Study:

  • To develop and validate a multiplex assay for the identification of peripheral blood using mRNA biomarkers.
  • To assess the sensitivity, specificity, and discriminatory power of the developed assay.
  • To evaluate the potential of mRNA single nucleotide polymorphisms (cSNPs) for forensic blood identification.

Main Methods:

  • Screened 14 cSNPs on four peripheral blood mRNA biomarkers (CD3G, ANK1, SPTB, GYPA).
Keywords:
Forensic peripheral blood identificationSNaPshot assaycSNPsmRNA

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Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
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Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Published on: September 16, 2012

Related Experiment Videos

Last Updated: Jul 13, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
14:45

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Published on: September 16, 2012

  • Developed a one-step multiplex reverse transcription PCR (RT-PCR) and SNaPshot assay for cDNA amplification and genotyping.
  • Assessed assay sensitivity, specificity, and capability on single-source and mixed body fluid samples.

    • Main Results:

    • The 14 cSNP panel successfully identified peripheral blood, including mixed samples.
    • The multiplex assay demonstrated a detection sensitivity of 1 ng total RNA.
    • Genotyping of 14 cSNP loci in 19 peripheral blood samples showed 100% concordance between DNA- and RNA-derived profiles.
    • The cumulative discrimination power of the 14 cSNPs was calculated to be 0.98303 in a Han Chinese population.

    Conclusions:

    • The developed multiplex SNaPshot assay is effective for forensic blood identification.
    • mRNA cSNPs can reliably differentiate peripheral blood from other body fluids like menstrual blood and vaginal secretions.
    • This assay provides a valuable tool for forensic analysis, enhancing the identification of blood evidence at crime scenes.