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CYP4B1 Expression in Breast Cancer: An Immunohistochemical Study with Complementary in silico Analyses.

Yousef M Al-Saraireh1, Fatemah O F O Alshammari2, Heba A Hassan1,3

  • 1Department of Pharmacology, Faculty of Medicine, University of Mutah, Al-Karak, Jordan.

Breast Cancer (Dove Medical Press)
|July 12, 2026
PubMed
Summary

Cytochrome 4B1 (CYP4B1) protein is found in a small subset of breast tumors, associated with lower grade. Bioinformatics analysis reveals reduced CYP4B1 mRNA in breast cancer, highlighting its role in xenobiotic metabolism.

Keywords:
Cytochrome 4B1breast cancerimmunohistochemistryproteomicsstructural modelingtranscriptomics

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Cytochrome 4B1 (CYP4B1) is an extrahepatic monooxygenase enzyme.
  • CYP4B1 expression has been noted in various malignancies.
  • Its specific role in breast cancer remains largely undetermined.

Purpose of the Study:

  • To investigate the expression and potential role of CYP4B1 in breast cancer.
  • To characterize CYP4B1 protein and mRNA levels in breast tumors.
  • To explore associations with clinical parameters and biological networks.

Main Methods:

  • Immunohistochemistry on a breast cancer tissue microarray (n=207 tumors, n=8 normal tissues).
  • In silico analyses including transcriptomic profiling, survival analysis, protein-protein interaction (PPI), functional enrichment, drug sensitivity correlation, and structural assessment using public databases.
  • AlphaFold structural assessment was utilized.

Main Results:

  • Focal and heterogeneous CYP4B1 protein expression detected in 18.3% of breast tumors, associated with lower histological grade.
  • No CYP4B1 expression observed in normal breast tissues.
  • Reduced CYP4B1 mRNA expression in breast cancer compared to normal tissue.
  • CYP4B1 identified within redox-regulatory and xenobiotic-metabolizing networks, including CYPs, GSTs, and SULTs.
  • No significant impact on survival; weak, descriptive correlations with drug sensitivity.

Conclusions:

  • CYP4B1 protein expression is limited and heterogeneous in breast cancer, linked to lower histological grade.
  • Bioinformatics analyses indicate reduced CYP4B1 mRNA expression and place it within key metabolic networks.
  • The prognostic and therapeutic significance of CYP4B1 in breast cancer requires further functional investigation.