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Published on: December 6, 2016
Research on Large Endothelial Cell-Derived Extracellular Vesicles in Pediatric Obstructive Sleep Apnea Syndrome
Weinan Lin1,2, Hezi Zhang3, Lichao Cao3
1Department of Respiratory Medicine, Shenzhen Children's Hospital, Shenzhen, Guangdong Province, People's Republic of China.
Insights
Large endothelial extracellular vesicles (EVs) are elevated in children with moderate-to-severe obstructive sleep apnea (OSA). These CD31+CD41- EVs correlate with OSA severity indicators like OAHI and reduced sleep efficiency, suggesting biomarker potential.
Area of Science:
- Cardiovascular Biology
- Sleep Medicine
- Extracellular Vesicles Research
Background:
- Obstructive sleep apnea (OSA) is a prevalent condition in children with potential long-term health consequences.
- Endothelial dysfunction is implicated in OSA pathogenesis.
- Extracellular vesicles (EVs) are emerging as potential biomarkers for various diseases.
Purpose of the Study:
- To investigate the association between clinical indicators of obstructive sleep apnea (OSA) and large endothelial cell-derived extracellular vesicles (large endothelial EVs) in children.
- To explore the potential of these EVs as biomarkers for OSA severity.
Main Methods:
- 81 children with OSA were enrolled (29 mild, 52 moderate-to-severe).
- Levels of CD31+CD41- and CD62E large endothelial EVs were measured.
- Receiver operating characteristic (ROC) curves assessed discriminatory ability for OSA severity.
- Spearman correlation and multiple regression analyses evaluated associations with clinical parameters (e.g., OAHI, sleep efficiency).
Main Results:
- CD31+CD41- large endothelial EVs were significantly elevated in children with moderate-to-severe OSA compared to mild OSA (p<0.05).
- ROC analysis showed CD31+CD41- EVs had good discriminatory power (AUC=0.848, sensitivity 82.8%, specificity 75%).
- CD31+CD41- EVs positively correlated with Obstructive Apnea-Hypopnea Index (OAHI) and negatively with sleep efficiency (p<0.05).
Conclusions:
- CD31+CD41- large endothelial EVs are closely associated with OSA severity indicators, including OAHI and sleep efficiency.
- These EVs show potential as a biomarker for assessing OSA severity in children.
- Further prospective validation is necessary for clinical application.
Purpose:
To explore the association between clinical indicators of obstructive sleep apnea (OSA) and large endothelial cell-derived extracellular vesicles (large endothelial EVs) in children, and to explore their potential association with disease severity.
Patients And Methods:
A total of 81 children with OSA were enrolled, including 29 cases of mild OSA and 52 cases of moderate-to-severe OSA. Receiver operating characteristic (ROC) curves evaluated the discriminatory ability of these EVs for severity groups. The correlation between large endothelial EVs and OSA clinical results, including sleep architecture and sleep-disordered breathing events, was evaluated using Spearman correlation analysis and multiple regression analysis.
Results:
Levels of CD31+CD41- large endothelial EVs were significantly elevated in children with moderate-to-severe OSA compared to the mild OSA group (p< 0.05). While CD62E levels were nominally elevated in moderate-to-severe OSA (p<0.05), this association did not persist following false discovery rate correction, and the marker showed limited predictive utility in the validation cohort (AUC = 0.538). The ROC curve results indicated that the sensitivity and specificity of CD31+CD41- in distinguishing mild OSA from moderate-to-severe OSA were 82.8% and 75%, respectively, with an area under the curve of 0.848. CD31+CD41- large endothelial EVs in children with OSA showed significant positive correlations with the Obstructive Apnea-Hypopnea Index (OAHI), hypopnea index, and oxygen desaturation index (p<0.05). Multiple regression analysis revealed a logarithmic positive correlation between OAHI and CD31+CD41- large endothelial EVs (p<0.01). Sleep efficiency was negatively correlated with CD31+CD41- large endothelial EVs (p<0.05).
Conclusion:
CD31+CD41- large endothelial EVs were closely associated with OAHI and sleep efficiency. These exploratory findings suggest their potential as a biomarker of OSA severity, but prospective validation is required before clinical application.
