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Published on: December 15, 2017
Critical Review on Microbial Inulinase Production: Emerging Strategies, AI-Driven Optimization, and Applications.
Sagar S Dhande1, Nikita A Mankoskar1, Harris P Panakkal1
1Department of Biotechnology, Government Institute of Science, Chhatrapati Sambhajinagar, Maharashtra, India.
Biotechnology and Bioengineering
|July 12, 2026
Summary
Microbial inulinases are key biocatalysts for sustainable production of valuable products. Innovations in enzyme engineering, AI-driven optimization, and nanomaterial immobilization address current bottlenecks like low yields and poor stability.
Area of Science:
- Biotechnology
- Enzymology
- Biocatalysis
Background:
- Microbial inulinases are vital biocatalysts for producing fructooligosaccharides, fructose, bioethanol, and organic acids.
- Research has advanced in strain selection, fermentation, enzyme engineering, and immobilization, yet bottlenecks like low yields and poor thermostability persist.
Purpose of the Study:
- To review strategies for overcoming bottlenecks in microbial inulinase production and application.
- To highlight advancements in immobilization, AI-driven optimization, and bioprospecting for improved enzyme performance and process efficiency.
Main Methods:
- Review of novel immobilization strategies using nanomaterials.
- Application of statistical and computational modeling (RSM, ANN, AI) for process optimization.
- Bioprospecting of extremophiles using metagenomics.
Main Results:
- Advanced immobilization techniques enhance operational stability and reusability for continuous processing.
- AI and computational modeling significantly increase yield and process efficiency in biorefinery applications.
- Novel strategies address limitations in yield, thermostability, and purification costs.
Conclusions:
- Overcoming bottlenecks requires a multidisciplinary approach integrating AI, economical immobilization, and circular bioeconomy principles.
- Future advancements necessitate AI-designed enzyme systems and valorization of agro-wastes for unlocking the bioeconomic potential of microbial inulinases.
- Resolving knowledge transfer barriers is crucial for the widespread adoption of microbial inulinase technology.
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