Related Experiment Video
Updated: Jul 14, 2026
11:51
Potato Virus X-Based microRNA Silencing (VbMS) In Potato.
Published on: May 11, 2020
The Transcription Factor DPB Confers Antiviral Defence Against Potato Virus X by Modulating MYB-Dependent Signalling
Jingjing Shi1, Yumei Zhao1, Chunyan Qi1
1State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China.
Molecular Plant Pathology
|July 13, 2026
Summary
Nicotiana benthamiana DPB protein acts as a transcription factor to enhance plant antiviral defense against potato virus X. It regulates NbMYB, which mediates defense via jasmonic acid and salicylic acid signaling pathways.
Area of Science:
- Plant molecular biology
- Plant pathology
- Transcriptional regulation
Background:
- DP proteins, as E2F co-factors, are known regulators of plant cell-cycle processes.
- The role of DP proteins in plant antiviral defense mechanisms is not well understood.
Purpose of the Study:
- To investigate the function of DP proteins in plant antiviral transcriptional regulation.
- To elucidate the role of NbDPB in Nicotiana benthamiana's defense against potato virus X (PVX).
Main Methods:
- Gene knockout and overexpression of NbDPB in Nicotiana benthamiana.
- Analysis of PVX accumulation in treated plants.
- Promoter binding assays and gene expression analysis of NbMYB.
- Hormone level quantification and marker gene expression analysis for jasmonic acid (JA) and salicylic acid (SA) pathways.
Main Results:
- Knockout of NbDPB increased PVX susceptibility, while overexpression suppressed viral accumulation.
- NbDPB directly binds to the NbMYB promoter and positively regulates its expression.
- Silencing NbMYB enhanced PVX infection, and JA/SA hormone treatments partially rescued this phenotype.
- JA and SA signaling pathways, along with their marker genes, were downregulated in NbMYB-silenced plants.
Conclusions:
- The NbDPB-NbMYB module plays a critical role in mediating antiviral defense in plants.
- This pathway involves the regulation of jasmonic acid and salicylic acid signaling.
- DP proteins are identified as key players in plant antiviral transcriptional regulation.
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