Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

¹⁹F MRI radiomic features: in vitro and in vivo repeatability.

European radiology experimental·2026
Same author

Nanoparticle ultrastructure allows reversible pH sensitivity using <sup>19</sup>F NMR and <i>in vivo</i> <sup>19</sup>F MRI.

Nanoscale advances·2026
Same author

Using tunable hydrogel microparticles to measure cellular forces.

Nature protocols·2025
Same author

Perfluorocarbon-Loaded Poly(lactide-<i>co</i>-glycolide) Nanoparticles from Core to Crust: Multifaceted Impact of Surfactant on Particle Ultrastructure, Stiffness, and Cell Uptake.

ACS applied polymer materials·2025
Same author

Polymeric (Poly(lactic-<i>co</i>-glycolic acid)) Particles Entrapping Perfluorocarbons Are Stable for a Minimum of Six Years.

ACS omega·2025
Same author

The internal structure of gadolinium and perfluorocarbon-loaded polymer nanoparticles affects <sup>19</sup>F MRI relaxation times.

Nanoscale·2023

Related Experiment Video

Updated: Jul 14, 2026

Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions
06:56

Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions

Published on: October 10, 2013

Monodisperse PEG engineering for quantifiable surface conjugation on PLGA nanoparticles.

Ezgi Basavci1,2, Alvja Mali1, Marjan Kalati1

  • 1Cell Biology and Immunology, Wageningen University and Research (WUR) Wageningen Netherlands mangala.srinivas@wur.nl.

Nanoscale Advances
|July 13, 2026
PubMed
Summary

This study engineered poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) using monodisperse polyethylene glycol (PEG) for improved stability and functionality. Monodisperse PEGylation offers precise control and enhanced properties for nanocarrier applications.

More Related Videos

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
06:47

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique

Published on: September 20, 2011

Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry
10:18

Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry

Published on: May 27, 2018

Related Experiment Videos

Last Updated: Jul 14, 2026

Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions
06:56

Particles without a Box: Brush-first Synthesis of Photodegradable PEG Star Polymers under Ambient Conditions

Published on: October 10, 2013

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
06:47

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique

Published on: September 20, 2011

Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry
10:18

Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry

Published on: May 27, 2018

Area of Science:

  • Materials Science
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Surface PEGylation enhances nanoparticle (NP) properties but is limited by polydisperse polyethylene glycol (PEG) derivatives.
  • Precise quantification of PEGylation efficiency and its impact on NP characteristics remains challenging.

Purpose of the Study:

  • To systematically engineer poly(lactic-co-glycolic acid) nanoparticles (PLGA NPs) using structurally defined, monodisperse PEG-diamine derivatives.
  • To quantify PEGylation efficiency and evaluate its effects on NP stability, functionality, and biological performance.
  • To establish a robust platform for rational nanocarrier design using monodisperse PEG engineering.

Main Methods:

  • Synthesis and purification of monodisperse PEG-diamine derivatives (PEG6, PEG26, PEG45).
  • Covalent attachment of PEG to PLGA NPs via EDC/NHS chemistry.
  • Quantification of PEGylation using 1H NMR and analysis of NP properties using TGA, DTGA, and DLS.

Main Results:

  • Monodisperse PEGylation enabled precise quantification of surface modification.
  • Higher molar mass PEGs enhanced thermal stability and colloidal stability in protein-rich environments.
  • PEGylated NPs showed improved encapsulation and maintained narrow size distribution over time.
  • Formulations demonstrated good cell viability in PBMC and RAW macrophage assays.

Conclusions:

  • Monodisperse PEG engineering provides reproducible and quantitative surface modification of PLGA NPs.
  • This approach facilitates the rational design of advanced polymeric nanocarriers.
  • The developed method is suitable for applications such as 19F MRI.