Bio-inspired polyphenol-polyelectrolyte WRAP system for geometrically controlled AAV delivery in diverse anatomical...

Area of Science:

• Biomaterials Science
• Drug Delivery Systems
• Gene Therapy

Background:

• Systemic drug delivery often leads to toxicity and low local concentrations.
• Localized delivery strategies aim to improve therapeutic efficacy at target sites.
• Existing methods face challenges in adhesion and retention on wet tissues.

Purpose of the Study:

• To develop a novel biomaterial scaffold for localized drug and gene delivery.
• To create a system that enhances therapeutic retention and efficacy at target sites.
• To minimize systemic toxicity associated with therapeutic interventions.

Main Methods:

• Development of a WRAP (Writable, Retentive, Adhesive, Permeable) scaffold using chitosan and alginate polyelectrolyte complex (PEC) films.
• Incorporation of chitosan-gallol (Chi-G) for enhanced adhesion and therapeutic loading.
• Application of the WRAP scaffold for adeno-associated virus (AAV) delivery.

Main Results:

• The WRAP scaffold demonstrated tunable film properties for maximized local therapeutic concentrations.
• Chitosan-gallol incorporation improved adhesion and retention on wet tissue surfaces.
• WRAP facilitated in situ viral retention, significantly improving adeno-associated virus (AAV) transgene delivery and prolonging expression.

Conclusions:

• The WRAP scaffold is a versatile platform for controlled, site-specific delivery of therapeutics.
• This system enhances spatial confinement, representing a precision medicine approach.
• WRAP offers a promising strategy to improve therapeutic outcomes while minimizing adverse events.