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Updated: Jul 14, 2026
Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
Published on: January 7, 2019
Evaluation of T-cell clonality by T-cell constant β chain (TRBC)1 and TRBC2 expression flow cytometry in
Alejandra Sánchez-Santos1,2, Alicia Puente-Fernández3, Davinia Godoy-Diaz3
1Respiratory Service, Hospital Universitario de Gran Canaria Dr. Negrín, Barranco de la Ballena s/n, 35019 Las Palmas de Gran Canaria, Spain.
Hypereosinophilic syndrome is a rare hematopoietic disorder characterized by persistently elevated hypereosinophilia in peripheral blood and tissues, leading to multiorgan damage. Hypereosinophilic syndrome can be classified into different subtypes based on diagnostic criteria and clinical characteristics: primary (neoplastic), secondary (reactive), idiopathic, and familial. Idiopathic is the most common subtype, characterized by systemic symptoms without an identifiable underlying cause. Diagnosis requires the exclusion of all primary and secondary causes of hypereosinophilia. Recently, T-cell receptor constant β chain 1 and T-cell receptor constant β chain 2 have emerged as a powerful tool for assessing T-cell clonality by flow cytometry. We evaluated T-cell receptor constant β chain 1 and T-cell receptor constant β chain 2 expression as a new diagnostic tool for detecting T-cell clonality in hypereosinophilic syndrome. Our findings were consistent with the results of molecular T-cell clonality testing. Flow cytometry is a rapid diagnostic method for assessing T-cell receptor constant β chain restriction and accurately determining the targetable T-cell receptor constant β chain isoform expressed by clonal T-cells in hypereosinophilic syndrome.
Hypereosinophilic syndrome is a rare hematopoietic disorder characterized by persistently elevated hypereosinophilia in peripheral blood and tissues, leading to multiorgan damage. Hypereosinophilic syndrome can be classified into different subtypes based on diagnostic criteria and clinical characteristics: primary (neoplastic), secondary (reactive), idiopathic, and familial. Idiopathic is the most common subtype, characterized by systemic symptoms without an identifiable underlying cause. Diagnosis requires the exclusion of all primary and secondary causes of hypereosinophilia. Recently, T-cell receptor constant β chain 1 and T-cell receptor constant β chain 2 have emerged as a powerful tool for assessing T-cell clonality by flow cytometry. We evaluated T-cell receptor constant β chain 1 and T-cell receptor constant β chain 2 expression as a new diagnostic tool for detecting T-cell clonality in hypereosinophilic syndrome. Our findings were consistent with the results of molecular T-cell clonality testing. Flow cytometry is a rapid diagnostic method for assessing T-cell receptor constant β chain restriction and accurately determining the targetable T-cell receptor constant β chain isoform expressed by clonal T-cells in hypereosinophilic syndrome.
