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Related Concept Videos

Adherens Junctions01:24

Adherens Junctions

Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
The endothelial cells...
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
Biofilms01:29

Biofilms

Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
The Oral Microbiota01:27

The Oral Microbiota

The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...

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Related Experiment Video

Updated: Jul 14, 2026

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
12:16

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Published on: December 17, 2015

Low DYNLL1 Resists Porphyromonas gingivalis-Induced Epithelial Jamming-Like State.

Q Li1, J Li1, D Tao1

  • 1State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China.

Journal of Dental Research
|July 13, 2026
PubMed
Summary

Impaired healing after periodontal therapy involves epithelial jamming. Targeting Dynein Light Chain LC8-Type 1 (DYNLL1) restores epithelial dynamics, improves junctional epithelium regeneration, and mitigates bone loss in periodontitis.

Keywords:
P. gingivalisbiophysicsdyneinsjunctional epitheliumperiodontal regenerationperiodontitis

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Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro
08:41

Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro

Published on: March 28, 2025

Related Experiment Videos

Last Updated: Jul 14, 2026

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
12:16

Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells

Published on: December 17, 2015

Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro
08:41

Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro

Published on: March 28, 2025

Area of Science:

  • Periodontal disease research
  • Epithelial biology
  • Wound healing mechanisms

Background:

  • Incomplete junctional epithelium (JE) regeneration post-periodontal therapy correlates with bone loss and disease recurrence.
  • Host-intrinsic factors governing epithelial repair after periodontal treatment remain poorly understood.
  • Porphyromonas gingivalis infection triggers pathological epithelial changes hindering JE reconstruction.

Purpose of the Study:

  • To investigate host-intrinsic mechanisms of impaired epithelial regeneration in periodontitis.
  • To identify key molecular regulators of epithelial biophysical properties during periodontal healing.
  • To explore therapeutic strategies targeting epithelial dynamics for enhanced periodontal repair.

Main Methods:

  • Genome-wide CRISPR-Cas9 screening to identify mediators of epithelial jamming.
  • Investigating the role of Dynein Light Chain LC8-Type 1 (DYNLL1) in epithelial cell adhesion and motility.
  • Utilizing a murine periodontitis model to assess the therapeutic potential of DYNLL1 modulation.

Main Results:

  • P. gingivalis challenge induces epithelial jamming, restricting remodeling necessary for JE repair.
  • DYNLL1 was identified as a critical mediator, regulating JUP accumulation and intercellular adhesion.
  • Partial DYNLL1 deficiency enhanced Wnt/β-catenin signaling, promoting epithelial responsiveness and repair.
  • Dynll1 haploinsufficiency in mice improved JE morphology, barrier function, and reduced bone loss.

Conclusions:

  • Aberrant epithelial biophysical properties, driven by DYNLL1, impede periodontal healing.
  • Modulating epithelial dynamics via DYNLL1 inhibition offers a complementary therapeutic approach to antimicrobial treatments.
  • Targeting host-intrinsic epithelial mechanisms presents a novel strategy for enhancing periodontal regeneration and preventing disease recurrence.