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Updated: Jul 14, 2026
Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
Published on: August 31, 2019
Preparation, Characterization, Optimization, and In vitro Anti-inflammatory Evaluation of Epigallocatechin Gallate
Neelesh Singh1,2, Rajesh Choudhary1, Shilpi Prasad2
1Shri Shankaracharya College of Pharmaceutical Sciences, Shri Shankaracharya Professional University, Bhilai, 490020, Chhattisgarh, India.
Introduction:
Sustained joint inflammation and oxidative stress are symptoms of Rheumatoid Arthritis (RA), an autoimmune disorder characterized by chronic inflammation. The present study was designed to prepare Epigallocatechin Gallate (EGCG)-loaded Nanostructured Lipid Carriers (NLCs) for enhanced therapeutic benefits in inflammatory conditions like RA.
Methods:
The NLCs were prepared using lecithin using a modified hot homogenizationultrasonication approach containing an aqueous-to-lipid phase ratio of 3:1 (v/v), where the lipid phase consisted of stearic acid and oleic acid (70:30 ratio), while the aqueous phase contained Tween 80 (2% w/v) and soy lecithin (1% w/v). The effect of drug concentration, lipid-to-drug ratio, or stirring time on particle size, zeta potential, and entrapment efficiency was investigated and optimized using a Box-Behnken design. Solubility profiles and partition coefficients were analyzed by spectrophotometric methods. The antioxidant and anti-inflammatory activity of the optimized EGCG-loaded NLCs was evaluated using an in vitro DPPH, H2O2, and protein denaturation assay as a preliminary screening model.
Results:
The optimum EGCG-NLCs showed 82.78% DPPH radical scavenging, 92% H2O2 radical scavenging, and 97.23% inhibition of protein denaturation at high concentration (100 µg/ml).
Discussion:
The antioxidant and anti-inflammatory activity of EGCG-NLCs demonstrated their therapeutic acceptability for the topical treatment of inflammatory conditions.
Conclusion:
In conclusion, EGCG-loaded NLCs demonstrated enhanced stability, bioactivity, and applicability for targeted inflammatory conditions such as rheumatoid arthritis therapy. This nanocarrier holds great promise as a phytochemical vehicle delivery system, which can also be integrated with other sophisticated drug delivery structures and undergo in vivo testing in the future.
