Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
Oogenesis02:07

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of hCG-to-oocyte retrieval interval on oocyte performances in women with polycystic ovary syndrome undergoing progestin-primed ovarian stimulation: a retrospective cohort trial.

Journal of assisted reproduction and genetics·2025
Same author

Comparable Pregnancy Loss and Neonatal Birthweights in Frozen Embryo Transfer Cycles Using Vitrified Embryos from Progestin-Primed Ovarian Stimulation and GnRH Analogue Protocols: A Retrospective Cohort Study.

Journal of clinical medicine·2022
Same author

Differences in Ectopic Pregnancy Rates between Fresh and Frozen Embryo Transfer after In Vitro Fertilization: A Large Retrospective Study.

Journal of clinical medicine·2022
Same author

Strengthened luteal phase support for patients with low serum progesterone on the day of frozen embryo transfer in artificial endometrial preparation cycles: a large-sample retrospective trial.

Reproductive biology and endocrinology : RB&E·2021
Same author

Elevated basal luteinizing hormone does not impair the outcome of human menopausal gonadotropin and medroxyprogesterone acetate treatment cycles.

Scientific reports·2018
Same author

[Kinase-Glo luminescent kinase assay for in vitro determination of PKA activity].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2012

Related Experiment Video

Updated: Jul 14, 2026

A Modified Technique for Inducing Polycystic Ovary Syndrome in Mice
04:49

A Modified Technique for Inducing Polycystic Ovary Syndrome in Mice

Published on: July 5, 2024

STEAP4-Mediated ROS-TERT-TP53 Signaling Promotes Granulosa Cell Dysfunction in Experimental Models of Polycystic

Xinxin Quan1,2,3, Xue Xue1,2,3, Huilan Ma1,2,3

  • 1Department of Cardiovascular Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.

Cells
|July 13, 2026
PubMed
Summary

Polycystic ovary syndrome (PCOS) involves granulosa cell apoptosis. This study reveals six-transmembrane epithelial antigen of prostate 4 (STEAP4) exacerbates PCOS by increasing oxidative stress and apoptosis, suggesting STEAP4 as a therapeutic target.

Keywords:
P53 pathwayPCOSSTEAP4TERToxidative stress

More Related Videos

Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model
09:44

Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model

Published on: March 5, 2022

Related Experiment Videos

Last Updated: Jul 14, 2026

A Modified Technique for Inducing Polycystic Ovary Syndrome in Mice
04:49

A Modified Technique for Inducing Polycystic Ovary Syndrome in Mice

Published on: July 5, 2024

Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model
09:44

Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model

Published on: March 5, 2022

Area of Science:

  • Endocrinology
  • Cell Biology
  • Reproductive Medicine

Background:

  • Polycystic ovary syndrome (PCOS) is a common endocrine disorder with unclear causes.
  • Increased granulosa cell apoptosis contributes to abnormal follicular development in PCOS.
  • The role of six-transmembrane epithelial antigen of prostate 4 (STEAP4) in PCOS-related granulosa cell dysfunction requires elucidation.

Purpose of the Study:

  • To investigate the function of STEAP4 in granulosa cells within PCOS models.
  • To explore the molecular mechanisms by which STEAP4 influences granulosa cell apoptosis and proliferation.
  • To assess the therapeutic potential of targeting STEAP4 in PCOS.

Main Methods:

  • Experimental PCOS models were established using KGN cells and C57BL/6 mice treated with dehydroepiandrosterone (DHEA).
  • STEAP4 expression, cell proliferation, and apoptosis were quantified using qRT-PCR, Western blot, CCK-8 assays, EdU labeling, and flow cytometry.
  • Mechanisms involving Nrf2 nuclear translocation, HIF-1α ubiquitination, and the ROS-TERT-TP53 axis were investigated.

Main Results:

  • Androgen exposure increased STEAP4 expression in both in vitro and in vivo PCOS models.
  • STEAP4 knockdown reduced proliferation and apoptosis in DHEA-treated granulosa cells.
  • STEAP4 stabilized HIF-1α, increased reactive oxygen species (ROS), promoted Nrf2 translocation, activated TERT and TP53 pathways, and alleviated PCOS symptoms in mice.

Conclusions:

  • STEAP4 promotes granulosa cell apoptosis and oxidative stress via the ROS-TERT-TP53 axis in PCOS.
  • STEAP4 plays a significant role in PCOS pathogenesis.
  • STEAP4 represents a potential therapeutic target for PCOS requiring further clinical investigation.