Drivers of Host-Pathogen Community Assemblages in European Forests and Urban Green Spaces

Despite advances in understanding infectious diseases, the persistence and re-emergence of wildlife pathogens continue to raise public and veterinary health concerns. This study investigates the relationship between biodiversity and rodent-borne diseases in Europe, focusing on habitat alterations and their impact on rodent diversity. We present host-pathogen data from 21 temperate forest sites and eight urban green spaces throughout five European countries, environments where rodents are abundant and human/domestic animals-wildlife interactions are likely to occur. From 2020 to 2022, 3766 specimens comprising 15 different small mammal species were analyzed. Samples were screened for bacteria via 16S rRNA sequencing or PCR, and for viral antibodies using immunofluorescent assays. Pathogens from several genera, including Bartonella, Borrelia, Mycoplasma, Anaplasma, Neoehrlichia, Leptospira, Orthohantavirus, and Orthopoxvirus, were detected at non-negligible prevalence in 11 host species. Host community composition differed between habitats, with more urban adapters in parks than in forests. Pathogen richness increased with an increase in host species diversity, supporting the "host-diversity begets parasite-diversity" hypothesis, though not with anthropization. The absence of some vector-transmitted parasites in urban areas suggests a shift in pathogen community driven by human impact. Host species and intrinsic factors were dominant explanatory variables for Mycoplasma species and Sarcocystidae, while extrinsic environmental and climatic factors influenced variations in several vector-transmitted pathogens. Apodemus sylvaticus and Clethrionomys glareolus served as important connector hosts in urban spaces and temperate forests, respectively. These results improve our understanding of the complex local host-pathogen system, aiding future management decisions and supporting the public health sector.