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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

Durum Wheat cv. Svevo Reference Genome Rel.2.0: A Comprehensive Tool for Wheat Genomics.

Elisabetta Mazzucotelli¹, Cristian Forestan², Gina Zastrow-Hayes³

¹Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria (CREA), Centro di ricerca Genomica e Bioinformatica, Fiorenzuola d'Arda, Italy.

Plant Biotechnology Journal

|July 13, 2026

Summary

A new durum wheat reference genome assembly (Svevo Rel.2.0) provides a highly contiguous and accurate sequence. This enhanced durum genome resource includes comprehensive annotation and a transcriptome atlas, facilitating genetic studies and trait discovery.

Keywords:

QTLome annotation de novo assembly domestication durum wheat (Triticum turgidum subsp. durum)haplotype‐based ancestry analysis long‐read sequencing transcriptome atlas

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Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

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Published on: July 16, 2019

Area of Science:

Plant Genomics
Wheat Genetics
Bioinformatics

Background:

Advancements in sequencing technologies enable high-quality plant genome assemblies.
Durum wheat (Triticum turgidum L. ssp. durum) is a vital global crop with a complex genome.

Purpose of the Study:

To produce a chromosome-level reference genome assembly for durum wheat (cv. Svevo).
To provide a comprehensive annotation and transcriptome atlas for the durum wheat genome.
To facilitate the identification of genes underlying important agronomic traits.

Main Methods:

Chromosome-level assembly using long-reads, optical mapping, and Hi-C technology.
Extensive annotation with short- and long-read RNA sequencing data.
Manual curation of storage protein and Leucine-Rich Repeat-Containing Receptor genes.
Mapping of quantitative trait loci (QTLs) and marker-trait associations (MTAs).

Main Results:

The Svevo Rel.2.0 assembly spans 10.4 Gb across 14 pseudomolecules with an N50 of 112.3 Mb.
Annotation identified 68,154 high-confidence protein-coding genes and 3763 LRR-CR loci.
Accurate reconstruction of the Tg1-B locus confirmed assembly integrity.
Mapping of 6621 QTLs/MTAs identified hotspots and candidate genes for trait discovery.
Haplotype analysis revealed a mosaic ancestry pattern for the durum genome.

Conclusions:

The Svevo Rel.2.0 represents the most comprehensive durum wheat reference genome to date.
The integrated transcriptome atlas and QTLome data offer powerful resources for durum wheat research.
This genome assembly will accelerate the understanding of wheat genetics and breeding for improved crop performance.