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Updated: Jul 15, 2026

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
Published on: September 2, 2019
Genome-wide data discovery of genetic variations in an advanced rice mutant line, NMR191, with blast resistance and
Wan Dalila Wan Chik1, Faiz Ahmad1,2, Wan Iryani Wan Ismail3
1Agrotechnology and Biosciences Division, Malaysian Nuclear Agency Bangi, 43000, Kajang, Selangor, Malaysia.
Abstract:
Climate change poses increasing challenges to rice production and food security, particularly in tropical regions such as Malaysia. This article presents a whole-genome resequencing dataset of a Malaysian-developed advanced rice mutant line, NMR191, and its parental cultivar, Pongsu Seribu (PS2), generated through ion beam irradiation-induced mutagenesis. Sequencing was performed using the Illumina NovaSeq X platform at approximately 30 × coverage, producing high-quality paired-end reads. Clean reads were aligned to the Oryza sativa Indica reference genome (GCA_000004655), followed by genome-wide identification of single nucleotide polymorphisms (SNPs) and insertions/deletions (InDels). The dataset comprises 24.8 GB and 28.6 GB of clean sequencing data for NMR191 and PS2, respectively. Raw sequencing reads are publicly available in the NCBI Sequence Read Archive under accession numbers and SRR36593642. This dataset provides a valuable genomic resource for variant discovery, comparative genomics, and future studies in rice breeding and mutation research.

