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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Chronic Obstructive Pulmonary Disease-II: Pathophysiology01:20

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

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Related Experiment Video

Updated: Jul 15, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

Integrated miRNA-mRNA network analysis identifies miR-182-5p as a potential regulator in COPD pathogenesis.

Hannah Burke1,2, Jodie Ackland1, Bastian R Angermann3

  • 1Faculty of Medicine, University of Southampton, Southampton, United Kingdom.

Frontiers in Medicine
|July 13, 2026
PubMed
Summary

Extracellular vesicle (EV)-derived microRNAs (miRNAs) show altered interactions with messenger RNAs (mRNAs) in chronic obstructive pulmonary disease (COPD). MiR-182-5p is a key regulator in these networks, potentially impacting COPD development.

Keywords:
extracellular vesiclesgene expression profilinggene expression regulationgene regulatory networkslung diseases, obstructivemiR-182-5pmicroRNAspulmonary disease, chronic obstructive

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CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
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Last Updated: Jul 15, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
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Published on: June 15, 2018

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

Area of Science:

  • Pulmonary Medicine
  • Molecular Biology
  • Genomics

Background:

  • Chronic obstructive pulmonary disease (COPD) is a major global health concern with limited therapeutic options.
  • Extracellular vesicles (EVs) facilitate intercellular communication via microRNAs (miRNAs), which are implicated in disease pathogenesis.
  • Previous work identified eight differentially expressed EV miRNAs in individuals with COPD compared to healthy controls.

Purpose of the Study:

  • To investigate miRNA-mRNA interactions within the lung epithelium of COPD patients.
  • To identify specific regulatory networks contributing to COPD pathogenesis.
  • To explore the role of previously identified EV-derived miRNAs in COPD.

Main Methods:

  • RNA sequencing of epithelial brushings from COPD patients and healthy ex-smokers.
  • In silico analysis to predict miRNA-mRNA interactions.
  • Network analysis and enrichment assessments to identify key regulatory pathways.
  • Correlation analysis between gene expression and CT-based disease probability measures.

Main Results:

  • 191 differentially expressed genes were identified in COPD lung epithelium.
  • 121 miRNA-mRNA interactions involving identified EV miRNAs were predicted.
  • miR-182-5p was identified as a central regulatory hub targeting multiple differentially expressed genes.
  • Gene expression changes correlated with CT-derived measures of emphysema and small airways disease.

Conclusions:

  • EV-derived miRNA-mRNA regulatory networks play a potential role in COPD pathogenesis.
  • miR-182-5p is a putative key regulator within these networks.
  • Exploratory findings suggest links to metabolic and immune processes, warranting further investigation.