Related Experiment Video
Updated: Jul 15, 2026
High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network
Published on: January 29, 2018
Multimodal imaging platform for rapid non-destructive evaluation of pancreatic puncture specimens
Jianan Zhang1, Qing Zhang1, Jiansheng Wang1
1East China Normal University, Shanghai Key Laboratory of Multidimensional Information Processing, Shanghai, China.
Significance:
Destructive staining and slow subjective visual inspection remain major limitations in rapid evaluation of biopsy specimens during endoscopic ultrasound-guided fine needle biopsy. Therefore, there is a strong need for a label-free optical imaging approach that enables quantitative specimen assessment while preserving specimen integrity.
Aim:
A multimodal imaging system integrating hyperspectral and color imaging was developed to acquire images of biopsy specimens without staining or sample preparation.
Approach:
A hyperspectral on-site evaluation workflow was proposed, integrating hyperspectral imaging and color imaging within a modular platform. Hyperspectral data were analyzed using a support-vector-machine-based segmentation framework for specimen assessment with color images providing reference annotations. No washing or staining is required.
Results:
Validation on 220 pancreatic biopsy specimens demonstrates accurate non-destructive macroscopic visible core evaluation, achieving an -score of 94.7%. The proposed workflow improves analysis efficiency by 93% compared with conventional rapid on-site evaluation.
Conclusions:
We demonstrate that integrating hyperspectral imaging with computational analysis enables rapid, quantitative, and label-free specimen evaluation, highlighting the potential of biomedical optical systems for intra-procedural specimen assessment.
