Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

Generation of Mosaic Mammary Organoids by Differential Trypsinization

Generation of Mosaic Mammary Organoids by Differential Trypsinization

Published on: March 11, 2020

A Defined Hydrogel-Based Method For Generating Three-Dimensional Human Breast Organoids That Recapitulate Mammary

Nicole Traugh¹, Meloryn Seraj¹, Gat Rauner²

¹Department of Developmental, Molecular & Chemical Biology, Tufts University School of Medicine.

Journal of Visualized Experiments : Jove

|July 13, 2026

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

COVID vaccination and post-infection cancer signals: Evaluating patterns and potential biological mechanisms.

Oncotarget·2026

Same author

Field cancerization, accelerated aging, and immunosuppression: the rapid rise of hormone-sensitive and early-onset breast cancer.

NPJ breast cancer·2025

Same author

Correction: Single-cell organogenesis captures complex breast tissue formation in three dimensions.

Development (Cambridge, England)·2025

Same author

Triple-Negative Breast Cancer Cells Activate Sensory Neurons via TRPV1 to Drive Nerve Outgrowth and Tumor Progression.

bioRxiv : the preprint server for biology·2025

Same author

Single-cell organogenesis captures complex breast tissue formation in three dimensions.

Development (Cambridge, England)·2025

Same author

From 2D to 3D and beyond: the evolution and impact of in vitro tumor models in cancer research.

Nature methods·2025

Researchers developed a new 3D human breast organoid model using a hydrogel matrix. This scalable system accurately mimics mammary gland development and can be used to study early breast cancer changes.

Area of Science:

Biomedical Engineering
Developmental Biology
Cancer Research

Background:

Studying human breast development and early carcinogenesis is challenging due to limitations in current model systems.
Conventional 2D cultures and many 3D systems do not fully replicate the mammary gland's structural organization and microenvironment.

Purpose of the Study:

To develop a reproducible, physiologically relevant 3D human breast organoid model.
To create a system that recapitulates mammary gland morphogenesis and supports early carcinogenesis studies.

Main Methods:

Generating 3D human breast organoids from primary epithelial cells within a defined hydrogel matrix (collagen, laminin, fibronectin, hyaluronic acid).
Culturing organoids for 21 days to observe key developmental stages.

More Related Videos

Generating and Imaging Mouse and Human Epithelial Organoids from Normal and Tumor Mammary Tissue Without Passaging

Generating and Imaging Mouse and Human Epithelial Organoids from Normal and Tumor Mammary Tissue Without Passaging

Published on: November 11, 2022

Related Experiment Videos

Last Updated: Jul 15, 2026

Generation of Mosaic Mammary Organoids by Differential Trypsinization

Generation of Mosaic Mammary Organoids by Differential Trypsinization

Published on: March 11, 2020

Generating and Imaging Mouse and Human Epithelial Organoids from Normal and Tumor Mammary Tissue Without Passaging

Generating and Imaging Mouse and Human Epithelial Organoids from Normal and Tumor Mammary Tissue Without Passaging

Published on: November 11, 2022

Utilizing high-content imaging for quantitative analysis of organoid characteristics.

Main Results:

The hydrogel-embedded organoid system successfully supported mammary morphogenesis, including progenitor expansion and terminal ductal lobular unit-like structure formation.
A mesenchyme-like compartment emerged within the organoids.
The system demonstrated compatibility with quantitative analysis of organoid development and complexity.

Conclusions:

This novel 3D human breast organoid platform provides a scalable and biologically relevant model for studying mammary gland development.
The system enables mechanistic investigations into epithelial plasticity and environmental factors influencing breast cancer risk.