Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 15, 2026

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation
07:20

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

Published on: August 30, 2017

Protocol for Standardized Assessment of Systemic Microvascular Function in Human Cutaneous Microcirculation Using

Ernesto Novaes1, Andrea De Lorenzo1, Eduardo Tibirica2

  • 1Research and Teaching Department, National Institute of Cardiology.

Journal of Visualized Experiments : Jove
|July 13, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond the Scale: Obesity as a Structural Driver of Refractory Hypertension.

Arquivos brasileiros de cardiologia·2026
Same author

Association between systemic microvascular dysfunction and resistant hypertension: Insights from a clinical observational study.

Microvascular research·2026
Same author

How to perform quantification of myocardial blood flow using single-photon emission computed tomography myocardial perfusion imaging.

Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology·2025
Same author

The association between cutaneous microvascular dysfunction and pulmonary artery hemodynamics in heart transplant candidates.

Microvascular research·2025
Same author

Perioperative microcirculatory monitoring using handheld video microscopy: a proof-of-concept observation.

Brazilian journal of anesthesiology (Elsevier)·2025
Same author

Redefining text-to-SQL metrics by incorporating semantic and structural similarity.

Scientific reports·2025

This study presents a standardized Laser Speckle Contrast Imaging (LSCI) protocol for assessing human skin microvascular function. The method uses controlled conditions and provocative tests to reliably measure blood flow and endothelial responses.

Area of Science:

  • Optical imaging
  • Physiology
  • Vascular biology

Background:

  • Microvascular dysfunction is implicated in various diseases.
  • Standardized, non-invasive methods are needed to assess microvascular health.
  • Laser Speckle Contrast Imaging (LSCI) offers real-time visualization of blood perfusion.

Purpose of the Study:

  • To present a standardized protocol for evaluating systemic microvascular function in human cutaneous microcirculation using LSCI.
  • To improve reproducibility and reliability of LSCI measurements by detailing strict standardization procedures.
  • To facilitate the adoption of standardized LSCI methodologies in clinical and translational research.

Main Methods:

  • Detailed environmental controls (temperature, acclimatization, minimal interference).

Related Experiment Videos

Last Updated: Jul 15, 2026

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation
07:20

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

Published on: August 30, 2017

  • Integration of LSCI with post-occlusive reactive hyperemia to assess integrated microvascular reactivity.
  • Iontophoresis-driven pharmacological challenges (Acetylcholine, sodium nitroprusside) to assess endothelial-dependent and -independent vasodilation.

    • Main Results:

    • The protocol ensures high sensitivity and reliability in LSCI measurements.
    • Successfully applied to identify microvascular impairment in conditions like resistant hypertension, diabetes, and coronary artery disease.
    • Demonstrates reproducible and non-invasive assessment of microvascular reactivity.

    Conclusions:

    • Standardized LSCI provides a valuable tool for investigating systemic vascular health.
    • Enables monitoring of disease progression and evaluation of interventions targeting the microvasculature.
    • Facilitates reproducible, non-invasive assessment of microvascular reactivity for clinical and translational research.