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Related Concept Videos

Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

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Related Experiment Video

Updated: Jul 15, 2026

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

Research Progress on DNA Damage Repair Efficiency Assessment Strategies.

Xiao Liu1, Yiwei Zhu2, Ling Li1

  • 1Jiangsu Province Engineering Research Center of Development and Translation of Key Technologies for Chronic Disease Prevention and Control, Suzhou Vocational Health College; School of Chemistry and Life Sciences, Suzhou University of Science and Technology.

Journal of Visualized Experiments : Jove
|July 13, 2026
PubMed
Summary

Genomic DNA damage is constant, but DNA repair mechanisms maintain stability and prevent cancer. This review covers major DNA repair pathways and methods to assess their efficiency for cancer therapy and drug development.

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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

Related Experiment Videos

Last Updated: Jul 15, 2026

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Cellular genomic DNA faces constant damage from internal and external sources, potentially reaching 10^4-10^5 lesions daily per cell.
  • Unrepaired DNA damage, especially during S phase, can cause mutations in critical genes, leading to cancer.
  • DNA repair is crucial for maintaining genomic stability and preventing cellular transformation.

Purpose of the Study:

  • To review major DNA damage repair pathways.
  • To discuss methods for assessing DNA repair capacity and efficiency.
  • To highlight the significance of DNA repair in cancer development and therapy.

Main Methods:

  • Literature review of major DNA damage repair pathways.
  • Discussion of functional assays for measuring DNA repair system activity.
  • Analysis of advantages and limitations of different assessment approaches.

Main Results:

  • Identification of key DNA damage repair pathways.
  • Overview of techniques to evaluate the functional activity of these pathways.
  • Comparison of the strengths and weaknesses of various DNA repair assessment methods.

Conclusions:

  • DNA repair efficiency is critical for genomic stability and cancer prevention.
  • Assessing DNA repair capacity is vital for developing chemotherapeutics and cancer treatments.
  • Understanding repair pathway efficiencies holds significant theoretical and practical implications.