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A Strategy to Identify Compounds that Affect Cell Growth and Survival in Cultured Mammalian Cells at Low-to-Moderate Throughput
Published on: September 22, 2019
High-throughput screening for cell death inducers and inhibitors
1Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, P.R. China.
Methods in Cell Biology
|July 13, 2026
Summary
High-throughput screening (HTS) identifies molecules that control programmed cell death (PCD). This method aids in discovering new treatments for diseases linked to cell death dysregulation, advancing drug development.
Area of Science:
- Cell Biology
- Pharmacology
- Biochemistry
Background:
- Programmed cell death (PCD) is vital for cellular homeostasis.
- Dysregulated PCD is linked to cancer, neurodegeneration, and autoimmune diseases.
- Understanding cell death mechanisms is crucial for disease treatment.
Purpose of the Study:
- To outline a protocol for using high-throughput screening (HTS) to identify modulators of programmed cell death (PCD).
- To discover novel small molecules and proteins that influence cell death pathways.
- To accelerate the identification of therapeutic candidates targeting abnormal cell death.
Main Methods:
- Utilizing cell-based assays and biochemical methods for rapid compound screening.
- Employing advanced data analysis techniques to identify active compounds.
- Integrating HTS with genome-wide screening approaches.
Main Results:
- HTS enables rapid identification of compounds that induce or inhibit key cell death processes.
- The protocol facilitates the discovery of novel small molecules and proteins modulating cell death.
- This approach provides insights into the molecular mechanisms of PCD.
Conclusions:
- HTS is an essential tool for studying programmed cell death (PCD).
- This protocol accelerates the discovery of therapeutic agents for diseases involving cell death.
- Further exploration is needed to fully understand cell death pathways and their therapeutic potential.
Keywords:
ATP-based viability assayAnnexin V / propidium iodide (PI) dual-fluorescence assayCell death inducers and inhibitorsHigh-throughput screening (HTS)Z′ factor
