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Chilling tolerance in kiwifruit alleviated by brassinosteroid: roles in ROS scavenging, membrane lipid stabilization,
Yupei Zhang1, Lin Shen2, Zhenyu Huang3
1Jiangxi Provincial Key Laboratory for Postharvest Storage and Preservation of Fruits & Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China.
Abstract:
This study examined the role of brassinosteroid (BR) application in mitigating chilling injury (CI) in 'Donghong' kiwifruit during cold storage. BR markedly alleviated CI symptoms, reduced lignification, electrolyte leakage, and malondialdehyde accumulation. It suppressed ROS buildup (O₂·- and H₂O₂) and enhanced antioxidant capacity through elevated activities and gene expression of SOD, CAT, and enzymes in the AsA-GSH cycle. BR also increased unsaturated fatty acid content, inhibited phospholipid degradation, and improved membrane integrity by modulating related enzyme activities and gene expression. Furthermore, BR maintained higher energy status, as indicated by increased ATP and ADP levels, energy charge, and enhanced activities of H+-ATPase, Ca2+-ATPase, CCO, and SDH, along with their up-regulated gene expression. These results demonstrate that BR enhances chilling tolerance by regulating ROS metabolism, membrane lipid stability, and energy metabolism.
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