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Related Experiment Video

Updated: Jul 15, 2026

Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene) Layers on Gold Microelectrodes for Uric Acid-Sensing Applications

Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene) Layers on Gold Microelectrodes for Uric Acid-Sensing Applications

Published on: July 28, 2021

Colorimetric correction of electrocatalytic urea quantification.

Tianshang Shan^1,2, Hongpan Rong^3,4, Zechao Zhuang⁵

¹School of Materials Science & Engineering, Beijing Institute of Technology, Beijing, 100081, China.

Nature Communications

|July 13, 2026

Summary

Accurate urea quantification is crucial for evaluating electrocatalyst performance in sustainable urea synthesis. A refined urease method accounts for ammonium impurities, ensuring reliable assessment of urea electrosynthesis catalysts.

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Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

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Published on: October 10, 2018

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Last Updated: Jul 15, 2026

Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene) Layers on Gold Microelectrodes for Uric Acid-Sensing Applications

Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene) Layers on Gold Microelectrodes for Uric Acid-Sensing Applications

Published on: July 28, 2021

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

Electrochemical Impedance Spectroscopy as a Tool for Electrochemical Rate Constant Estimation

Published on: October 10, 2018

Area of Science:

Electrochemistry
Catalysis
Analytical Chemistry

Background:

Electrochemical C-N coupling offers a green route for urea synthesis.
Efficient electrocatalysts are vital for high catalytic performance.
Accurate urea quantification is essential for assessing catalyst efficiency.

Purpose of the Study:

To address the issue of false-positive urea quantification caused by urease impurities.
To develop a reliable method for assessing urea electrosynthesis catalysts.
To validate the refined quantification method in a CO2/NO3- system.

Main Methods:

Investigated the presence of ammonium (NH4+) impurities in commercial urease enzymes.
Developed a modified urease method by incorporating a subtractive term for NH4+ impurities.
Validated the refined method using a previously reported CO2/NO3- electrochemical system.

Main Results:

All tested commercial ureases contained significant NH4+ impurities.
The presence of NH4+ impurities led to false-positive urea concentration readings.
The refined urease method accurately quantified urea by accounting for NH4+ impurities.

Conclusions:

Standard urease-based urea quantification methods are unreliable due to inherent NH4+ impurities.
A simple subtractive correction for NH4+ impurities ensures accurate urea quantification.
This refined method supports reliable development and assessment of urea electrosynthesis technologies.