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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jul 15, 2026

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

Reconfigurable single-shot spectroscopy reaching 86 femtometers resolution.

Tianwei Jiang¹, Jie Li², Lu Chen²

¹State Key Laboratory of Information Photonics and Optical Communications, Beijing University of Posts and Telecommunications, Beijing, China. jtw@bupt.edu.cn.

Nature Communications

|July 13, 2026

Summary

A new reconfigurable single-shot spectroscopy method overcomes limitations in spectral bandwidth, resolution, and speed. This breakthrough achieves record spectral resolution, enabling detailed analysis of rapid transient events.

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Last Updated: Jul 15, 2026

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

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Area of Science:

Optical Spectroscopy
Photonics
Instrumentation

Background:

Single-shot spectroscopy captures transient events but faces trade-offs between spectral bandwidth, resolution, and acquisition speed.
Existing systems limit versatility and adoption due to inherent incompatibilities in performance parameters.

Purpose of the Study:

To introduce a reconfigurable single-shot spectroscopy method with programmable control over spectral bandwidth, resolution, and acquisition speed.
To overcome the traditional limitations in spectral resolution and enhance the versatility of single-shot spectroscopic techniques.

Main Methods:

Developed a reconfigurable single-shot spectroscopy system offering full programmability.
Demonstrated programmable navigation of the spectral bandwidth, resolution, and acquisition speed trade-off.
Achieved a record spectral resolution of 86 femtometers in the 1.5 μm band.

Main Results:

Successfully captured rapid dynamic spectral fluctuations of phase-shift fiber Bragg gratings.
Recorded responses to both continuous (9 kHz) and sudden (0.1 ms) vibrations.
Achieved spectral resolution comparable to state-of-the-art Fourier transform spectrometers.

Conclusions:

The reconfigurable single-shot spectroscopy method broadens the application landscape for analyzing rapid, non-repetitive processes.
This innovation paves the way for the commercialization of advanced single-shot spectroscopy.
Enables precise measurement of dynamic spectral changes in optical devices.