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Correction: Kubiak, K.; Inkielewicz-Stępniak, I. Epigenetic Regulators as Therapeutic Targets in Pancreatic Ductal Adenocarcinoma. <i>Cancers</i> 2026, <i>18</i>, 1001.

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Related Experiment Video

Updated: Jul 15, 2026

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
05:17

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Published on: May 14, 2019

Exploring platelet-covered and naked circulating tumor cells - a single-cell transcriptomic perspective.

Michał Sieczczyński1,2, Krzysztof Pastuszak1,3,4, Marcin Banacki1,5

  • 1Laboratory of Translational Oncology, Intercollegiate Faculty of Biotechnology of the University of Gdańsk and the Medical University of Gdańsk, Gdańsk, Poland.

Journal of Translational Medicine
|July 14, 2026
PubMed
Summary

Platelet-covered circulating tumor cells (CTCs) were identified and analyzed. Platelet cloaking significantly impacts CTC survival and metastatic progression, offering new insights into liquid biopsy.

Keywords:
Circulating tumor cellsPlatelet-covered circulating tumor cellsSingle-cell sequencingepithelial–mesenchymal transition

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Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

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Last Updated: Jul 15, 2026

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
05:17

Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Published on: May 14, 2019

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
10:12

Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

Published on: May 15, 2018

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Circulating tumor cells (CTCs) and platelets can be collected during liquid biopsy.
  • The interaction between CTCs and platelets, forming platelet-covered CTCs (pcCTCs), is not fully understood.
  • Understanding pcCTCs is crucial for advancing liquid biopsy techniques and cancer diagnostics.

Purpose of the Study:

  • To detect and characterize pcCTCs using single-cell data from peripheral blood mononuclear cells (PBMCs).
  • To compare the transcriptomic profiles of pcCTCs with naked CTCs and platelets.
  • To investigate the influence of platelet cloaking on CTC behavior and metastatic potential.

Main Methods:

  • Analysis of 22 patient samples (10 breast cancer, 12 ovarian cancer) and 7 control samples.
  • Identification of candidate CTCs and pcCTCs using single-cell data.
  • Transcriptomic analysis of identified CTCs and platelets, focusing on EMT, angiogenesis, and platelet-related pathways.

Main Results:

  • 12 candidate CTCs were identified, including 5 naked CTCs and 7 pcCTCs.
  • Pathways related to epithelial-mesenchymal transition (EMT), angiogenesis, and platelet activation were upregulated in pcCTCs.
  • Platelet-associated signals were highest in pcCTCs, with distinct scores for epithelial/mesenchymal phenotypes and cancer-specific gene sets.

Conclusions:

  • Platelet cloaking significantly alters gene expression in CTCs.
  • This alteration influences CTC survival in the bloodstream and contributes to metastatic progression.
  • Findings highlight the importance of considering platelet-CTC interactions in liquid biopsy and cancer research.