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Related Experiment Video

Updated: Jul 15, 2026

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish
09:27

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Published on: April 1, 2021

Integrative multi-omics analysis implicates the RNF40-LIMA1 axis in hepatocellular carcinoma progression and immune

Zhan Liu1, Shaobo Wu2, Kexin Fan2

  • 1School of Basic Medical Sciences, Xinjiang Second Medical College, Karamay, Xinjiang, China.

Frontiers in Oncology
|July 14, 2026
PubMed
Summary

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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...

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Ring Finger Protein 40 (RNF40) targets LIM domain and actin-binding protein 1 (LIMA1) for degradation, suppressing Hepatocellular Carcinoma (HCC) progression. This study elucidates the RNF40-LIMA1 axis, offering potential therapeutic targets for HCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Ubiquitin Biology

Background:

  • Ring Finger Protein 40 (RNF40), an E3 ubiquitin ligase, has context-dependent roles in cancer.
  • RNF40 targets LIM domain and actin-binding protein 1 (LIMA1) for degradation.
  • The RNF40-LIMA1 interaction in Hepatocellular Carcinoma (HCC) is not well understood, despite resistance to immune checkpoint inhibitors.

Purpose of the Study:

  • To investigate the molecular mechanisms of RNF40-mediated LIMA1 ubiquitination in HCC progression.
  • To explore the functional relationship between RNF40 and LIMA1 in HCC.
  • To identify potential therapeutic targets for HCC, considering resistance to current therapies.

Main Methods:

  • Integrative multi-omics analysis (bulk transcriptomics, scRNA-seq, spatial transcriptomics).
Keywords:
LIMA1RNF40hepatocellular carcinomaspatial transcriptomicstumor microenvironmentubiquitination

Related Experiment Videos

Last Updated: Jul 15, 2026

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish
09:27

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Published on: April 1, 2021

  • Experimental validation using in vitro assays and in vivo mouse models.
  • Differential expression, survival analysis, immune infiltration, and cell-cell communication analyses.

    • Main Results:

    • LIMA1 is significantly overexpressed in HCC, correlating with increased proliferation, migration, invasion, and an immunosuppressive tumor microenvironment (TME).
    • LIMA1 overexpression promotes HCC progression, while RNF40 overexpression suppresses it; these effects are dependent on LIMA1 levels.
    • LIMA1's prognostic value in HCC requires cautious interpretation and further validation.

    Conclusions:

    • A functional RNF40-LIMA1 axis is biologically relevant in HCC progression.
    • This study provides a rationale for investigating the RNF40-LIMA1 axis as a therapeutic target in HCC.
    • Further validation of LIMA1's prognostic and immunotherapy-predictive value in independent cohorts is needed.