Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 15, 2026

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish
09:50

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish

Published on: April 10, 2020

A Phenotype-Driven Discovery of Pro-Revascularization Chalcone Derivatives Using Zebrafish and CAM Models.

Yau-Hung Chen1, Biswajit Mohanty2, Tao-Sheng Li3

  • 1Department of Chemistry, Tamkang University, New Taipei City, Taiwan, tku.edu.tw.

Journal of Toxicology
|July 14, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydrostatic pressure activates HIF-1α via β-catenin to promote stemness in breast cancer cells.

FEBS open bio·2026
Same author

Computational investigation of covalent organic pyrgos[<i>n</i>]cages as potential dual modulators of the VEGFR-2 D2-D3 domain and DNA structural interfaces.

Physical chemistry chemical physics : PCCP·2026
Same author

Hydrogen-Bond Topology Governs Clustering-Triggered Emission in Non-Conjugated Small Molecules.

The journal of physical chemistry. B·2026
Same author

Zebrafish <i>PRL-3</i> Regulates Yolk Syncytial Layer Integrity and Actomyosin Contractility During Epiboly.

International journal of molecular sciences·2026
Same author

Effect of Short-Term and Long-Term Non-Physiological T3 Concentrations on Cardiac Stromal Cells: From Cellular Response to In Vivo Adaptation.

Medical sciences (Basel, Switzerland)·2026
Same author

A Metal-Free Phthalocyanine Additive for Defect Passivation and Processing Tolerance in High-Efficiency Perovskite Solar Cells.

Small (Weinheim an der Bergstrasse, Germany)·2026

Novel chalcone derivative 1c significantly enhances blood vessel formation, offering a promising proangiogenic agent for treating vascular diseases. Computational analysis supports its superior activity and potential as a therapeutic lead.

Area of Science:

  • Medicinal Chemistry
  • Molecular Biology
  • Computational Chemistry

Background:

  • Angiogenesis is vital for tissue repair and treating ischemic diseases, but effective small-molecule proangiogenic agents are scarce.
  • Chalcone derivatives are recognized for their diverse biological activities, making them candidates for therapeutic development.

Purpose of the Study:

  • To synthesize and evaluate the proangiogenic potential of novel chalcone derivatives.
  • To elucidate the structure-activity relationship (SAR) of these compounds using computational chemistry.

Main Methods:

  • Synthesis and in vivo testing of six chalcone derivatives (1a-1f) in zebrafish models (Tg(fli1:egfp)).
  • Assessment of vascular network formation using the chick embryo chorioallantoic membrane (CAM) assay.
Keywords:
SARchalconechickpro-angiogenicreal-time PCRzebrafish

More Related Videos

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model
10:23

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Published on: November 13, 2012

Large Scale Zebrafish-Based In vivo Small Molecule Screen
07:03

Large Scale Zebrafish-Based In vivo Small Molecule Screen

Published on: December 30, 2010

Related Experiment Videos

Last Updated: Jul 15, 2026

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish
09:50

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish

Published on: April 10, 2020

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model
10:23

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Published on: November 13, 2012

Large Scale Zebrafish-Based In vivo Small Molecule Screen
07:03

Large Scale Zebrafish-Based In vivo Small Molecule Screen

Published on: December 30, 2010

  • Computational chemistry analyses including Density Functional Theory (DFT), Molecular Electrostatic Potential (MESP), and Frontier Molecular Orbital (FMO) theory.

    • Main Results:

    • Compound 1c demonstrated the highest proangiogenic activity, significantly increasing vessel branching and outgrowth in zebrafish.
    • Compound 1c promoted caudal vein plexus remodeling and increased intercapillary spaces in transgenic zebrafish.
    • Gene expression analysis revealed 1c upregulates cadherin 5 and neuropilin 1a while downregulating fms-related receptor tyrosine kinase 1.
    • Computational analyses correlated compound 1c's high electrophilicity and electron transfer capability with its potent biological activity.

    Conclusions:

    • Chalcone derivative 1c exhibits potent proangiogenic activity, validated through in vivo and in vitro assays.
    • The study provides a strong correlation between computational chemical properties and biological efficacy.
    • Compound 1c represents a promising lead for developing novel small-molecule therapeutics for vascular dysfunction diseases.