Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Polymer Classification: Crystallinity01:21

Polymer Classification: Crystallinity

Unlike ionic or small covalent molecules, polymers do not form crystalline solids due to the diffusion limitations of their long-chain structures. However, polymers contain microscopic crystalline domains separated by amorphous domains.
Crystalline domains are the regions where polymer chains are aligned in an orderly manner and held together in proximity by intermolecular forces. For example, chains in the crystalline domains of polyethylene and nylon are bound together by van der Waals...
Cell Inclusions01:27

Cell Inclusions

Prokaryotic cells possess a variety of inclusions that play crucial roles in nutrient storage, metabolic processes, and environmental adaptation. These structures enable bacteria to thrive under fluctuating environmental conditions by storing essential resources and optimizing their metabolic efficiency.Carbon Storage: Poly-β-Hydroxybutyric Acid and Glycogen GranulesBacteria frequently store excess carbon in specialized granules. Poly-β-hydroxybutyric acid (PHB) granules are lipid polymers that...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-canonical function of transferrin receptor-1 promotes breast cancer metastasis by activating HCK‒STAT3‒MMP9 signalling.

Clinical and translational medicine·2026
Same author

Structural basis for prohibitin-mediated regulation of mitochondrial m-AAA protease.

Nature communications·2026
Same author

Discovery and characterization of YSA64, a RBM39 degrader with in vivo efficacy and potent cellular activity in pediatric Ewing sarcoma A673.

European journal of medicinal chemistry·2026
Same author

rRNA expansion segments mediate ribosome dimerization as a conserved stress response.

Nucleic acids research·2026
Same author

CGG Repeat Expansion in GIPC1 is Associated with Childhood-Onset Hereditary Ataxia.

Movement disorders : official journal of the Movement Disorder Society·2026
Same author

Discovery of Novel MLKL PROTAC Degraders for the Treatment of Hepatocellular Carcinoma via Promoting Parthanatos.

Journal of medicinal chemistry·2026

Related Experiment Video

Updated: Jul 15, 2026

Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
09:06

Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography

Published on: November 20, 2021

Structural Polymorphism of polyG Inclusions Revealed by In Situ Cryo-Electron Tomography.

Yunwen Qian1, Yalan Wan2,3, Chen Chen1,4

  • 1State Key Laboratory of Membrane Biology, Peking-Tsinghua Joint Center For Life Sciences, Academy for Advanced Interdisciplinary Studies, School of Life Sciences, Peking University, Beijing, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|July 14, 2026
PubMed
Summary

Neuronal intranuclear inclusion disease (NIID) involves polyG inclusions with unique ribbon structures, not amyloid fibrils. These structures interact with cellular components, impacting cell function and offering insights into NIID pathogenesis.

Keywords:
cryo‐ETneurodegenerationneuronal intranuclear inclusion diseasepolyG diseases

More Related Videos

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography
09:47

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography

Published on: July 17, 2018

Related Experiment Videos

Last Updated: Jul 15, 2026

Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
09:06

Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography

Published on: November 20, 2021

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography
09:47

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography

Published on: July 17, 2018

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Neuronal intranuclear inclusion disease (NIID) is characterized by intranuclear inclusions.
  • The precise ultrastructure of these inclusions, particularly polyG aggregates, remains poorly understood.

Purpose of the Study:

  • To elucidate the native ultrastructure of polyG inclusions in NIID.
  • To investigate the in situ interactions of these inclusions with cellular compartments.

Main Methods:

  • Correlative cryo-electron tomography was employed in primary cortical neurons and NIID mouse models.
  • Ultrastructural analysis focused on the architecture of polyG aggregates and their cellular context.

Main Results:

  • PolyG inclusions form interconnected ribbon-like assemblies, distinct from amyloid fibrils.
  • Multiple compartment-specific ribbon states were identified, varying in proteasome accessibility.
  • Cytoplasmic ribbon assemblies interact with endomembranes, correlating with ER-stress gene dysregulation.

Conclusions:

  • The study reveals a core feature of polyG aggregation involves diverse ribbon states.
  • This provides an in situ framework for connecting NIID inclusion architecture to cellular dysfunction.
  • Findings offer new perspectives on the molecular mechanisms underlying NIID.